SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Enzyme Information

General Information
Standard nameUFD4
Systematic nameYKL010C
DescriptionUbiquitin-protein ligase (E3) that interacts with Rpt4p and Rpt6p, two subunits of the 19S particle of the 26S proteasome; cytoplasmic E3 involved in the degradation of ubiquitin fusion proteins.

Ubiquitination Features
Num. of substrates Total 2 wild-type known substrates. (1 synthetic substrate information)
Wild-type substrate
KeywordDescription
RAD4DNA repair protein RAD4
UBC7Ubiquitin-conjugating enzyme E2-18 kDa
Synthetic substrate information
UFD4 is involved in the polyubiquitylation of ubiquitin fusion protein UbV76-V-beta-galactosidase and UbV76-V-DHFRha.  [7615550] [12447385]

Cross References
Swiss-Prot accessionP33202
SGD YKL010C
Gene Ontology (GO)
Cellular componentGO:0005739mitochondrionIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Molecular functionGO:0004842ubiquitin-protein ligase activityTAS:SGD.
Biological processGO:0006513protein monoubiquitinationTAS:SGD.
Biological processGO:0000209protein polyubiquitinationTAS:SGD.
EC number

Additional Features
Post Translational Modifications (PTM)
Residue indexType
91Phosphoserine.

Sequence Information
Sequence length1483
Molecular weight167843

	---------+----------+----------+----------+----------+
MSENNSHNLD EHESHSENSD YMMDTQVEDD YDEDGHVQGE YSYYPDEDED   50
EHMLSSVGSF EADDGEDDDN DYHHEDDSGL LYGYHRTQNG SDEDRNEEED   100
GLERSHDNNE FGSNPLHLPD ILETFAQRLE QRRQTSEGLG QHPVGRTLPE   150
ILSMIGGRME RSAESSARNE RISKLIENTG NASEDPYIAM ESLKELSENI   200
LMMNQMVVDR IIPMETLIGN IAAILSDKIL REELELQMQA CRCMYNLFEV   250
CPESISIAVD EHVIPILQGK LVEISYIDLA EQVLETVEYI SRVHGRDILK   300
TGQLSIYVQF FDFLTIHAQR KAIAIVSNAC SSIRTDDFKT IVEVLPTLKP   350
IFSNATDQPI LTRLVNAMYG ICGALHGVDK FETLFSLDLI ERIVQLVSIQ   400
DTPLENKLKC LDILTVLAMS SDVLSRELRE KTDIVDMATR SFQHYSKSPN   450
AGLHETLIYV PNSLLISISR FIVVLFPPED ERILSADKYT GNSDRGVISN   500
QEKFDSLVQC LIPILVEIYT NAADFDVRRY VLIALLRVVS CINNSTAKAI   550
NDQLIKLIGS ILAQKETASN ANGTYSSEAG TLLVGGLSLL DLICKKFSEL   600
FFPSIKREGI FDLVKDLSVD FNNIDLKEDG NENISLSDEE GDLHSSIEEC   650
DEGDEEYDYE FTDMEIPDSV KPKKISIHIF RTLSLAYIKN KGVNLVNRVL   700
SQMNVEQEAI TEELHQIEGV VSILENPSTP DKTEEDWKGI WSVLKKCIFH   750
EDFDVSGFEF TSTGLASSIT KRITSSTVSH FILAKSFLEV FEDCIDRFLE   800
ILQSALTRLE NFSIVDCGLH DGGGVSSLAK EIKIKLVYDG DASKDNIGTD   850
LSSTIVSVHC IASFTSLNEF LRHRMVRMRF LNSLIPNLTS SSTEADREEE   900
ENCLDHMRKK NFDFFYDNEK VDMESTVFGV IFNTFVRRNR DLKTLWDDTH   950
TIKFCKSLEG NNRESEAAEE ANEGKKLRDF YKKREFAQVD TGSSADILTL   1000
LDFLHSCGVK SDSFINSKLS AKLARQLDEP LVVASGALPD WSLFLTRRFP   1050
FLFPFDTRML FLQCTSFGYG RLIQLWKNKS KGSKDLRNDE ALQQLGRITR   1100
RKLRISRKTI FATGLKILSK YGSSPDVLEI EYQEEAGTGL GPTLEFYSVV   1150
SKYFARKSLN MWRCNSYSYR SEMDVDTTDD YITTLLFPEP LNPFSNNEKV   1200
IELFGYLGTF VARSLLDNRI LDFRFSKVFF ELLHRMSTPN VTTVPSDVET   1250
CLLMIELVDP LLAKSLKYIV ANKDDNMTLE SLSLTFTVPG NDDIELIPGG   1300
CNKSLNSSNV EEYIHGVIDQ ILGKGIEKQL KAFIEGFSKV FSYERMLILF   1350
PDELVDIFGR VEEDWSMATL YTNLNAEHGY TMDSSIIHDF ISIISAFGKH   1400
ERRLFLQFLT GSPKLPIGGF KSLNPKFTVV LKHAEDGLTA DEYLPSVMTC   1450
ANYLKLPKYT SKDIMRSRLC QAIEEGAGAF LLS                     1483