SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Enzyme Information

General Information
Standard nameSSM4
Systematic nameYIL030C
AliasDOA10, KIS3
DescriptionUbiquitin-protein ligase of the ER/nuclear envelope, required for degradation of Alpha2p and other proteins containing a Deg1 degradation signal; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation.

Ubiquitination Features
Num. of substrates Total 2 wild-typeand 4 mutant known substrates. (3 synthetic substrate information)
Wild-type substrate
KeywordDescription
HMLALPHA2Mating-type protein ALPHA2
UBC6Ubiquitin-conjugating enzyme E2 6
Mutant substrate
KeywordWild type description
mCBF2Centromere DNA-binding protein complex CBF3 subunit A
mMPS2Uncharacterized protein YGL075C
mPMA1Plasma membrane ATPase 1
mSTE6Mating factor A secretion protein STE6
Synthetic substrate information
SSM4 ubiquitylates SL17/CL degron fusions in concert with UBC6 and UBC7. These fusions are beta-galactosidase-SL17, URA3-SL17, and URA3-CL1,2,6,9,10,11.  [16437165]
SSM4 ubiquitylates Deg1 fusion protein, Deg1-VP (Deg1 to N-terminus of VMA12) in concert with UBC6 and UBC7.  [16437165]
SSM4 ubiquitylates KSS (KHN luminal/STE6 transmembrane domains/STE6-166 cytosolic mutant domain) and KWS(KHN luminal domain/WSC1p transmembrane domain/STE6-166p mutant cytosolic domain) in concert with UBC7. The ubiquitylation of these substrates results in proteasomal degradation of them.  [15078901]

Cross References
Swiss-Prot accessionP40318
SGD YIL030C
Gene Ontology (GO)
Cellular componentGO:0030176integral to endoplasmic reticulum membraneIDA:SGD.
Cellular componentGO:0005637nuclear inner membraneIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Molecular functionGO:0004842ubiquitin-protein ligase activityIDA:SGD.
Biological processGO:0030433ER-associated protein catabolic processIMP:SGD.
EC number 6.3.2.-

Additional Features
Post Translational Modifications (PTM) PTM sites are not determined.

Sequence Information
Sequence length1319
Molecular weight151455

	---------+----------+----------+----------+----------+
MDVDSDVNVS RLRDELHKVA NEETDTATFN DDAPSGATCR ICRGEATEDN   50
PLFHPCKCRG SIKYMHESCL LEWVASKNID ISKPGADVKC DICHYPIQFK   100
TIYAENMPEK IPFSLLLSKS ILTFFEKARL ALTIGLAAVL YIIGVPLVWN   150
MFGKLYTMML DGSSPYPGDF LKSLIYGYDQ SATPELTTRA IFYQLLQNHS   200
FTSLQFIMIV ILHIALYFQY DMIVREDVFS KMVFHKIGPR LSPKDLKSRL   250
KERFPMMDDR MVEYLAREMR AHDENRQEQG HDRLNMPAAA ADNNNNVINP   300
RNDNVPPQDP NDHRNFENLR HVDELDHDEA TEEHENNDSD NSLPSGDDSS   350
RILPGSSSDN EEDEEAEGQQ QQQQPEEEAD YRDHIEPNPI DMWANRRAQN   400
EFDDLIAAQQ NAINRPNAPV FIPPPAQNRA GNVDQDEQDF GAAVGVPPAQ   450
ANPDDQGQGP LVINLKLKLL NVIAYFIIAV VFTAIYLAIS YLFPTFIGFG   500
LLKIYFGIFK VILRGLCHLY YLSGAHIAYN GLTKLVPKVD VAMSWISDHL   550
IHDIIYLYNG YTENTMKHSI FIRALPALTT YLTSVSIVCA SSNLVSRGYG   600
RENGMSNPTR RLIFQILFAL KCTFKVFTLF FIELAGFPIL AGVMLDFSLF   650
CPILASNSRM LWVPSICAIW PPFSLFVYWT IGTLYMYWFA KYIGMIRKNI   700
IRPGVLFFIR SPEDPNIKIL HDSLIHPMSI QLSRLCLSMF IYAIFIVLGF   750
GFHTRIFFPF MLKSNLLSVP EAYKPTSIIS WKFNTILLTL YFTKRILESS   800
SYVKPLLERY WKTIFKLCSR KLRLSSFILG KDTPTERGHI VYRNLFYKYI   850
AAKNAEWSNQ ELFTKPKTLE QAEELFGQVR DVHAYFVPDG VLMRVPSSDI   900
VSRNYVQTMF VPVTKDDKLL KPLDLERIKE RNKRAAGEFG YLDEQNTEYD   950
QYYIVYVPPD FRLRYMTLLG LVWLFASILM LGVTFISQAL INFVCSFGFL   1000
PVVKLLLGER NKVYVAWKEL SDISYSYLNI YYVCVGSVCL SKIAKDILHF   1050
TEGQNTLDEH AVDENEVEEV EHDIPERDIN NAPVNNINNV EEGQGIFMAI   1100
FNSIFDSMLV KYNLMVFIAI MIAVIRTMVS WVVLTDGILA CYNYLTIRVF   1150
GNSSYTIGNS KWFKYDESLL FVVWIISSMV NFGTGYKSLK LFFRNRNTSK   1200
LNFLKTMALE LFKQGFLHMV IYVLPIIILS LVFLRDVSTK QIIDISHGSR   1250
SFTLSLNESF PTWTRMQDIY FGLLIALESF TFFFQATVLF IQWFKSTVQN   1300
VKDEVYTKGR ALENLPDES                                     1319