SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Enzyme Information

General Information
Standard nameUBC6
Systematic nameYER100W
AliasDOA2
DescriptionUbiquitin-conjugating enzyme involved in ER-associated protein degradation; located at the cytosolic side of the ER membrane; tail region contains a transmembrane segment at the C-terminus; substrate of the ubiquitin-proteasome pathway.

Ubiquitination Features
Num. of substrates Total 1 wild-typeand 7 mutant known substrates.
Wild-type substrate
KeywordDescription
HMLALPHA2Mating-type protein ALPHA2
Mutant substrate
KeywordWild type description
mCBF2Centromere DNA-binding protein complex CBF3 subunit A
mMPS2Uncharacterized protein YGL075C
mPDR5Pleiotropic ABC efflux transporter of multiple drugs
mPMA1Plasma membrane ATPase 1
mPRC1Carboxypeptidase Y [Precursor]
mSEC61Protein transport protein SEC61
mSTE6Mating factor A secretion protein STE6

Cross References
Swiss-Prot accessionP33296
SGD YER100W
Gene Ontology (GO)
Cellular componentGO:0005789endoplasmic reticulum membraneIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Molecular functionGO:0004842ubiquitin-protein ligase activityIDA:SGD.
Biological processGO:0030433ER-associated protein catabolic processIGI:SGD.
Biological processGO:0006513protein monoubiquitinationTAS:SGD.
Biological processGO:0000209protein polyubiquitinationTAS:SGD.
EC number 6.3.2.19

Additional Features
Post Translational Modifications (PTM)
Residue indexType
178Phosphothreonine.

Sequence Information
Sequence length250
Molecular weight28383

	---------+----------+----------+----------+----------+
MATKQAHKRL TKEYKLMVEN PPPYILARPN EDNILEWHYI ITGPADTPYK   50
GGQYHGTLTF PSDYPYKPPA IRMITPNGRF KPNTRLCLSM SDYHPDTWNP   100
GWSVSTILNG LLSFMTSDEA TTGSITTSDH QKKTLARNSI SYNTFQNVRF   150
KLIFPEVVQE NVETLEKRKL DEGDAANTGD ETEDPFTKAA KEKVISLEEI   200
LDPEDRIRAE QALRQSENNS KKDGKEPNDS SSMVYIGIAI FLFLVGLFMK   250