SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Enzyme Information

General Information
Standard nameCDC34
Systematic nameYDR054C
AliasDNA6, UBC3
DescriptionUbiquitin-conjugating enzyme (E2) and catalytic subunit of SCF ubiquitin-protein ligase complex (together with Skp1p, Rbx1p, Cdc53p, and an F-box protein) that regulates cell cycle progression by targeting key substrates for degradation.

Ubiquitination Features
Num. of substrates Total 25 wild-type known substrates.
Wild-type substrate
KeywordDescription
AAH1Adenosine deaminase
CDC4Cell division control protein 4
CDC6Cell division control protein 6
CLB6S-phase entry cyclin-6
CLN1G1/S-specific cyclin CLN1
CLN2G1/S-specific cyclin CLN2
CTF13Centromere DNA-binding protein complex CBF3 subunit C
CYK2Cytokinesis 2 protein
FAR1Cyclin-dependent kinase inhibitor FAR1
GCN4General control protein GCN4
GIC2GTPase-interacting component 2
GRR1SCF E3 ubiquitin ligase complex F-box protein GRR1
HAC1Transcriptional activator HAC1
HOHomothallic switching endonuclease
IME2Meiosis induction protein kinase IME2/SME1
MET4Transcriptional activator of sulfur metabolism MET4
RCY1Recyclin-1
SIC1Protein SIC1
STD1Protein STD1
STE7Serine/threonine-protein kinase STE7
SWE1Mitosis inhibitor protein kinase SWE1
TEC1Ty transcription activator TEC1
UFO1Ubiquitin ligase complex F-box protein UFO1
YDR306CF-box protein YDR306C
YNL311CF-box protein YNL311C

Cross References
Swiss-Prot accessionP14682
SGD YDR054C
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Cellular componentGO:0005634nucleusIDA:SGD.
Cellular componentGO:0019005SCF ubiquitin ligase complexIDA:SGD.
Molecular functionGO:0042803protein homodimerization activityIDA:SGD.
Molecular functionGO:0004842ubiquitin-protein ligase activityIDA:SGD.
Biological processGO:0000082G1/S transition of mitotic cell cycleTAS:SGD.
Biological processGO:0000086G2/M transition of mitotic cell cycleIGI:SGD.
Biological processGO:0051865protein autoubiquitinationIDA:SGD.
Biological processGO:0000209protein polyubiquitinationIDA:SGD.
Biological processGO:0042787protein ubiquitination during ubiquitin-dep...IDA:SGD.
Biological processGO:0031146SCF-dependent proteasomal ubiquitin-depende...IDA:SGD.
EC number 6.3.2.19

Additional Features
Post Translational Modifications (PTM) PTM sites are not determined.

Sequence Information
Sequence length295
Molecular weight34065

	---------+----------+----------+----------+----------+
MSSRKSTASS LLLRQYRELT DPKKAIPSFH IELEDDSNIF TWNIGVMVLN   50
EDSIYHGGFF KAQMRFPEDF PFSPPQFRFT PAIYHPNVYR DGRLCISILH   100
QSGDPMTDEP DAETWSPVQT VESVLISIVS LLEDPNINSP ANVDAAVDYR   150
KNPEQYKQRV KMEVERSKQD IPKGFIMPTS ESAYISQSKL DEPESNKDMA   200
DNFWYDSDLD DDENGSVILQ DDDYDDGNNH IPFEDDDVYN YNDNDDDDER   250
IEFEDDDDDD DDSIDNDSVM DRKQPHKAED ESEDVEDVER VSKKI        295