SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Enzyme Information

General Information
Standard nameDOA4
Systematic nameYDR069C
AliasDOS1, MUT4, NPI2, SSV7, UBP4
DescriptionUbiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole.

Ubiquitination Features
Num. of substrates Total 7 wild-typeand 1 mutant known substrates.
Wild-type substrate
KeywordDescription
CPS1Carboxypeptidase S
GAL2Galactose transporter
GAP1General amino-acid permease GAP1
PUT4Proline-specific permease
SNA3Protein SNA3
STE6Mating factor A secretion protein STE6
TAT2Tryptophan permease
Mutant substrate
KeywordWild type description
mPMA1Plasma membrane ATPase 1

Cross References
Swiss-Prot accessionP32571
SGD YDR069C
Gene Ontology (GO)
Cellular componentGO:0005768endosomeIDA:SGD.
Cellular componentGO:0005624membrane fractionIDA:SGD.
Cellular componentGO:0005739mitochondrionIDA:SGD.
Cellular componentGO:0000502proteasome complex (sensu Eukaryota)IPI:SGD.
Molecular functionGO:0004175endopeptidase activityIGI:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Molecular functionGO:0004221ubiquitin thiolesterase activityIDA:SGD.
Biological processGO:0006897endocytosisIMP:SGD.
Biological processGO:0016579protein deubiquitinationTAS:SGD.
Biological processGO:0006275regulation of DNA replicationIMP:SGD.
Biological processGO:0000723telomere maintenanceIMP:SGD.
Biological processGO:0006511ubiquitin-dependent protein catabolic processIMP:SGD.
EC number 3.1.2.15

Additional Features
Post Translational Modifications (PTM) PTM sites are not determined.

Sequence Information
Sequence length926
Molecular weight105192

	---------+----------+----------+----------+----------+
MEQNIISTIR DECIRHRSKY LTIAQLTAIA EAKINEFIIT GKAKDQDLSS   50
LLDKCIDILS IYKKNSKDIK NIISCKNKGA MISSNSVMII QLNYVYYKVI   100
HIIVTTNIPH LSEFAKIKLH KSTSDEGNGN NNNNEFQLMN IYNTLLETLL   150
KDENIAKIKS FIKSSIKQTK LNHEQEECNL MRTGSYITSN QLNSLISSSA   200
NSASSQMEIL LIDIRSRLEF NKSHIDTKNI ICLEPISFKM SYSDHDLEKK   250
SLITSPNSEI KMFQSRNLFK FIILYTDANE YNVKQQSVLL DILVNHSFEK   300
PISDDFTKIF ILESGFPGWL KSNYGRQVSS SFPSNNNIKD DSVYINGNTS   350
GLSLQHLPKM SPSIRHSMDD SMKEMLVAPT PLNHLQQQQQ QQSDNDHVLK   400
RSSSFKKLFS NYTSPNPKNS NSNLYSISSL SISSSPSPLP LHSPDPVKGN   450
SLPINYPETP HLWKNSETDF MTNQREQLNH NSFAHIAPIN TKAITSPSRT   500
ATPKLQRFPQ TISMNLNMNS NGHSSATSTI QPSCLSLSNN DSLDHTDVTP   550
TSSHNYDLDF AVGLENLGNS CYMNCIIQCI LGTHELTQIF LDDSYAKHIN   600
INSKLGSKGI LAKYFARLVH MMYKEQVDGS KKISISPIKF KLACGSVNSL   650
FKTASQQDCQ EFCQFLLDGL HEDLNQCGSN PPLKELSQEA EARREKLSLR   700
IASSIEWERF LTTDFSVIVD LFQGQYASRL KCKVCSHTST TYQPFTVLSI   750
PIPKKNSRNN ITIEDCFREF TKCENLEVDE QWLCPHCEKR QPSTKQLTIT   800
RLPRNLIVHL KRFDNLLNKN NDFVIYPFLL DLTPFWANDF DGVFPPGVND   850
DELPIRGQIP PFKYELYGVA CHFGTLYGGH YTAYVKKGLK KGWLYFDDTK   900
YKPVKNKADA INSNAYVLFY HRVYGV                             926