SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameZRT1
Systematic nameYGL255W
DescriptionZinc-regulated transporter 1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1K195MONO, MULTIUBC4, UBC5HECTNARSP5  Vacuolar Degradation
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidenceUb-conjugate Confirmation
MethodWestern blot
Reference"Zinc-regulated ubiquitin conjugation signals endocytosis of the yeast ZRT1 zinc transporter."
Gitan RS, Eide DJ.
Biochem J. 346 Pt 2:329-36 (2000) [10677350]
[2]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP32804
SGD YGL255W
Gene Ontology (GO)
Cellular componentGO:0005887integral to plasma membraneIMP:SGD.
Molecular functionGO:0000006high affinity zinc uptake transporter activityIMP:SGD.
Biological processGO:0006830high-affinity zinc ion transportIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length376
Molecular weight41582

	---------+----------+----------+----------+----------+
MSNVTTPWWK QWDPSEVTLA DKTPDDVWKT CVLQGVYFGG NEYNGNLGAR   50
ISSVFVILFV STFFTMFPLI STKVKRLRIP LYVYLFAKYF GSGVIVATAF   100
IHLMDPAYGA IGGTTCVGQT GNWGLYSWCP AIMLTSLTFT FLTDLFSSVW   150
VERKYGLSHD HTHDEIKDTV VRNTAAVSSE NDNENGTANG SHDTKNGVEY   200
YEDSDATSMD VVQSFQAQFY AFLILEFGVI FHSVMIGLNL GSVGDEFSSL   250
YPVLVFHQSF EGLGIGARLS AIEFPRSKRW WPWALCVAYG LTTPICVAIG   300
LGVRTRYVSG SYTALVISGV LDAISAGILL YTGLVELLAR DFIFNPQRTK   350
DLRELSFNVI CTLFGAGIMA LIGKWA                             376

	* Ubiquitinated lysine is denoted as red color.