SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Systematic nameYPL158C
DescriptionUncharacterized protein YPL158C

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI    RPN10 Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionQ99299
SGD YPL158C
Gene Ontology (GO)
Cellular componentGO:0005935cellular bud neckIDA:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length758
Molecular weight84846

	---------+----------+----------+----------+----------+
MIIRAPIRTK TKSFRGDQMD FKFPSNESLP RGTLEEYHLN NHHLLNDVFA   50
AENGVSRDED GNSQTLSDYT STSNTNTNSG YSSNGYYSFA NISDNTTSSP   100
RIVINQNETA RLTSSDSNKS DFFASHDFPG NDSLHYSSSN VVKNQLHSME   150
AIPEGNITGS ISTAFQTIPT ADNVSYDIAP SSASSLLPRK STSKSAILPS   200
TQEAKPMTKL NMEKDIKTIE LNNSVVPKPK KKLNRVPTIR RVESSRFSNS   250
RYSSSVSSKS SSSRCSLKRS KAIRCKGGLL YYFTSLGIKI KKKLRKLRLV   300
LRRRLFSYNV QKVPSATNSK TTKSKANINN KSKKRGTNLV NKNSNSTPRQ   350
KKSQRYVSNL QRSISSKSLV PVLAPQKKTK PLTVDTKFKA NHPQSEDSKV   400
GSNTPRSPLV SYTPSLRRTN SSIRRAASIL TASATMTPAN NKNSFISVPD   450
NVSHAVTRNS SMYSRSRLVR SKPSTALNAI ARQPSIVVEN KVIPLSMNRY   500
SIKEEDEYVI DTSSMRELSP VNSVCSSDYD RESSESYSNY ADAMETTEVD   550
NKDRVECNNE IQNVDANNEE TSNEESYNLM KHYLSTVIAQ RIMLRVQIAR   600
IQNYKSNVVY MNKSAETNST IYEDLVDSLL TEYEADGSSS QIFDGVTVRA   650
DEEEEEDEDD EDDEEEEEEN DDEEDEEDEE DDEDDEEEEE KRKEGEGRNL   700
AKEVDELAEL SPMRKQSDLS ITLRSPFAML NPAYSNSIIS LPTGVVKRSL   750
TLPVGMKI                                                 758