SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


Main Search Enzymes Substrates Ubiquitination types BLAST Feedback HELP

Substrate Information

General Information
Systematic nameYOL045W
DescriptionProbable serine/threonine-protein kinase YOL045W

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI    RPN10 Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]

Cross References
Swiss-Prot accessionQ08217
SGD YOL045W
Gene Ontology (GO)
Cellular componentGO:0005739mitochondrionIDA:SGD.
Molecular functionGO:0004674protein serine/threonine kinase activityIDA:SGD.
Biological processGO:00060781,6-beta-glucan biosynthetic processIGI:SGD.
Biological processGO:0019318hexose metabolic processIGI:SGD.
Biological processGO:0006468protein amino acid phosphorylationIDA:SGD.
EC number 2.7.11.1

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
434Phosphoserine.

Sequence Information
Sequence length1101
Molecular weight124373

	---------+----------+----------+----------+----------+
MTYPVSAAAP ADISYSKNTP LVGLSKPPCL YQHASSSVDS FSSTFSDDDR   50
SDLVAVPNES PHAFSYNPIS PNSLGVRLTI LRRSLEIMVN SPDILHELKK   100
KAPVIAYPPS LRHTRNLTET ATLSASRDPL NGSLISPLVS NMPSPASRPV   150
IQRATSLMVL PDNDTASKLN PAKSELENLL FLLNLALENN SFERASDLHM   200
LSLLNIKKIN FDSDIQKSET LKKVLLDSLA EPFFENYKKF PHKDLGSKSQ   250
YNEYEEKHDD IVSLADIKPQ QDYSRILHPF TSAKNSGPEA IFTCSQQYPW   300
NFKAANDLAC LTFGISKNVI KALTLLDLIH TDSRNFVLEK IMNAEDDNQE   350
IVFTGETIPI VQPNSTSNNN VPNLIWASLW AKRKNGLLVC VFEKTPCDYI   400
DVMLNLRDFS VDSIIDTTHF LENFDKKKQQ ESTSPMTEKK TVKFANEIHD   450
IGSVSHSLSK LIDDVRFGKV FSADDDLLPL SIRVANHVNE ERYFTLNCLS   500
ENIPCAVTTS VLENEIKLKI HSLPYQAGLF IVDSHTLSLL SFNKSVAKNM   550
FGLRLHELAG SSVTKLVPSL ADMISYINKT YPMLNITLPE NKGLVLTEHF   600
FRKIEAEMHH DKDSFYTSIG LDGCHKDGNL IKVDVQLRVL NTNAVLLWIT   650
HSRDVVIENY TTVPSQLPML KENEIDVVGS RGSSSASSKK SSEKIPVNTL   700
KAMADLSISS AETISNSDDE VDLNQVNEKL RETSCGKVRG IESNDNNNYD   750
DDMTMVDDPE LKHKIELTKM YTQDKSKFVK DDNFKVDEKF IMRIIEPING   800
EEIKKETNEL DKRNSTLKAT YLTTPEANIG SQKRIKKFSD FTILQVMGEG   850
AYGKVNLCIH NREHYIVVIK MIFKERILVD TWVRDRKLGT IPSEIQIMAT   900
LNKNSQENIL KLLDFFEDDD YYYIETPVHG ETGSIDLFDV IEFKKDMVEH   950
EAKLVFKQVV ASIKHLHDQG IVHRDIKDEN VIVDSHGFVK LIDFGSAAYI   1000
KSGPFDVFVG TMDYAAPEVL GGSSYKGKPQ DIWALGVLLY TIIYKENPYY   1050
NIDEILEGEL RFDKSEHVSE ECISLIKRIL TREVDKRPTI DEIYEDKWLK   1100
I                                                        1101