SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


Main Search Enzymes Substrates Ubiquitination types BLAST Feedback HELP

Substrate Information

General Information
Systematic nameYNL321W
DescriptionPutative cation exchanger YNL321W

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP42839
SGD YNL321W
Gene Ontology (GO)
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
26Phosphothreonine.
110Phosphoserine.
120Phosphoserine.
121Phosphoserine.

Sequence Information
Sequence length908
Molecular weight102499

	---------+----------+----------+----------+----------+
MAKNNHISAS GNSTSGDHRL KEEVLTPTTS ASTPHRIFSV DDDPKEIQND   50
IRYLEGLHEG LKFALHANKS KRSVSSQSPI VHSSNNTLHH HEHQQHLPPT   100
LESLSSKSHS VPDLNTATPS SPKRMHSSIR ELPHDDNDDE DANDDSRFII   150
HDSHGHDLLI DEINCQSPSH LENNDQASNA SSTESFTLRE RQDAINETHP   200
FGIRIWKPAL YKKHRSVQRT AAQDIHETQL KTITWEVTCS NVLWFILFGF   250
PIAILFYSAA IVVFLLGGGG LVTNSAKEYS KCLYKLANYF LWPFGKMVYL   300
LQDEQYLQED KDEGISMQQF YNWVTSYSNR LVFHQSQAKF QQREDHPAPA   350
TESSSLMPPA NTTATPLNSN HPSYNSIRHE IPHAAAQRRY FGRGKWSWGR   400
VLFYTIFHLV LQPILAVLSL CLWLLVFTIP MSNVLWQIMY HCRRHPLALG   450
FKYVENSSQS HENEITQQQL NKNILLCTFR AAGWHYYKYT VDGTNVIVVN   500
LISIVFFTIF DFYVLKNFLH WKTWFTYESS IFILCLTSTI PLAFYIGQAV   550
ASISAQTSMG VGAVINAFFS TIVEIFLYCV ALQQKKGLLV EGSMIGSILG   600
AVLLLPGLSM CGGALNRKTQ RYNPASAGVS SALLIFSMIV MFVPTVLYEI   650
YGGYSVNCAD GANDRDCTFS HPPLKFNRLF THVIQPMSIS CAIVLFCAYI   700
IGLWFTLRTH AKMIWQLPIA DPTSTAPEQQ EQNSHDAPNW SRSKSTCILL   750
MSTLLYAIIA EILVSCVDAV LEDIPSLNPK FLGLTIFALI PNTTEFLNAI   800
SFAIHGNVAL SMEIGSAYAL QVCLLQIPSL VIYSIFYTWN VKKSMINIRT   850
QMFPLVFPRW DIFGAMTSVF MFTYLYAEGK SNYFKGSMLI LLYIIIVVGF   900
YFQGALSE                                                 908