SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Systematic nameYKR021W
DescriptionUncharacterized protein YKR021W

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1   HECTNARSP5   
2        Proteasomal Degradation
3         
* Blank: not determined, NA: not applicable.

References
[1]EvidenceUb-conjugate Confirmation
Methodprotein microarray (in vitro)
Reference"Ubiquitination screen using protein microarrays for comprehensive identification of Rsp5 substrates in yeast."
Gupta R, Kus B, Fladd C, Wasmuth J, Tonikian R, Sidhu S, Krogan NJ, Parkinson J, Rotin D.
Mol Syst Biol. 3:116 (2007) [17551511]
[2]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]
[3]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP36117
SGD YKR021W
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length915
Molecular weight102540

	---------+----------+----------+----------+----------+
MLQFNTENDT VAPVFPMEQD INAAPDAVPL VQTTTLQVFV KLAEPIVFLK   50
GFETNGLSEI APSILRGSLI VRVLKPNKLK SISITFKGIS RTEWPEGIPP   100
KREEFSDVET VVNHTWPFYQ ADDGMNSFTL EHHSSNNSSN RPSMSDEDYL   150
LEKSGASVYI PPTAEPPKDN SNLSLDAYER NSLSSDNLSN KPVSSDVSHD   200
DSKLLAIQKT PLPSSSRRGS VPANFHGNSL SPHTFISDLF TKTFSNSGAT   250
PSPEQEDNYL TPSKDSKEVF IFRPGDYIYT FEQPISQSYP ESIKANFGSV   300
EYKLSIDIER FGAFKSTIHT QLPIKVVRLP SDGSVEETEA IAISKDWKDL   350
LHYDVVIFSK EIVLNAFLPI DFHFAPLDKV TLHRIRIYLT ESMEYTCNSN   400
GNHEKARRLE PTKKFLLAEH NGPKLPHIPA GSNPLKAKNR GNILLDEKSG   450
DLVNKDFQFE VFVPSKFTNS IRLHPDTNYD KIKAHHWIKI CLRLSKKYGD   500
NRKHFEISID SPIHILNQLC SHANTLLPSY ESHFQYCDED GNFAPAADQQ   550
NYASHHDSNI FFPKEVLSSP VLSPNVQKMN IRIPSDLPVV RNRAESVKKS   600
KSDNTSKKND QSSNVFASKQ LVANIYKPNQ IPRELTSPQA LPLSPITSPI   650
LNYQPLSNSP PPDFDFDLAK RGAADSHAIP VDPPSYFDVL KADGIELPYY   700
DTSSSKIPEL KLNKSRETLA SIEEDSFNGW SQIDDLSDED DNDGDIASGF   750
NFKLSTSAPS ENVNSHTPIL QSLNMSLDGR KKNRASLHAT SVLPSTIRQN   800
NQHFNDINQM LGSSDEDAFP KSQSLNFNKK LPILKINDNV IQSNSNSNNR   850
VDNPEDTVDS SVDITAFYDP RMSSDSKFDW EVSKNHVDPA AYSVNVASEN   900
RVLDDFKKAF REKRK                                         915