SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Systematic nameYJL149W
DescriptionF-box protein YJL149W

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP47005
SGD YJL149W
Gene Ontology (GO)
Cellular componentGO:0019005SCF ubiquitin ligase complexIPI:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length663
Molecular weight77367

	---------+----------+----------+----------+----------+
MPFQDYFQKK KAAFINRNNK SNADASALRD INDININFAA KSKNYVFPLT   50
KLPDELMQEV FSHLPQPDRL QLCLVNKRLN KIATKLLYRR IYLNDSNVVK   100
SDFMHLAINW TLLNLPSSLK EEESRDIANC KLKKLIETLQ NNIHITEVIQ   150
WIRINWDLDS TLQRSILSIL CNQGKSLQRL ENVTDPACND IISNGHFSRS   200
NVSSFDMAPP NSLPEMVVPE NYIPNLTKYL SQRISSRLSH MTLFIDPLKL   250
FNYLYPLDIK LQIIDLKLHW RREFYNNDYF VKKIRPGNPL TKLSEVFDKR   300
TLKILTIISW NDTLLKRETE MLKDFKEFEN LEDLSLISIK QDVHILVDLF   350
SSLTNLKRLK MDFLEEYVPE PTNPHIFLSI LLACSKLQFI DLRYDGLIPQ   400
IINIQENKFQ LNQQCNCTNC QIVFSDILKG KIFMFPEDYY IHDLQDIAAK   450
DIFKMMKYLS LLPYSKACDA YPSVRTQPMN LTNFVTKMNR NLLEYRNSKS   500
QLVPKIVNNP HQHSTVTSTS TAHMSEPEMI IIDDDDDDDE INAAIPPSSD   550
DTAATISTDL ELPHESLTKR DIIMCYHALI HHFKSIYVTF LKSFPHLRFL   600
MLNDIPTIVM EENNERIFEP VFYHYDYKSN LYGWSKESNK NLENDSNNNN   650
NNSDTIARIA TVM                                           663