SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Systematic nameYJL084C
DescriptionUncharacterized protein YJL084C

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
2   HECTNARSP5   
3         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[2]EvidenceUb-conjugate Confirmation
Methodprotein microarray (in vitro)
Reference"Ubiquitination screen using protein microarrays for comprehensive identification of Rsp5 substrates in yeast."
Gupta R, Kus B, Fladd C, Wasmuth J, Tonikian R, Sidhu S, Krogan NJ, Parkinson J, Rotin D.
Mol Syst Biol. 3:116 (2007) [17551511]
[3]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP47029
SGD YJL084C
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Molecular functionGO:0030332cyclin bindingIDA:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
155Phosphoserine.
162Phosphoserine.
166Phosphoserine.
172Phosphoserine.
213Phosphoserine.
838Phosphoserine.
882Phosphoserine.
883Phosphoserine.
884Phosphothreonine.
900Phosphoserine.
1022Phosphoserine.
1023Phosphoserine.

Sequence Information
Sequence length1046
Molecular weight117216

	---------+----------+----------+----------+----------+
MPMDQSISSP LFPMEKDIDI PLDATPLAQS SSLQLFIHLA EPVVFLQGFD   50
PQKTEYPSVV LRGCLVVRIL KPTKLKSISL SFKGYSRTEW PEGIPPKRQE   100
FVEIKDIVDH TWALYPPTEQ KSKKKMDASA PNESNNAANN FLTKESGASL   150
YRTLSDNETI TSRKNSISGL SSLNLSPLGA PGNSSVNVKD RESRQRSRSS   200
SVTSSNGPSR NLSPINLLKR ATSPSVSHHN YKPTTTSIFS DLLNNTFTHN   250
DAASHHGHHI PTSSNHLAMT SNNFTSGSGG EFFVFQPGDY IYAFEELIPQ   300
AYPESIKADF GFVEYFLFAS IERPGAFKSN ISARQVVNIV RTQAHNSVEE   350
SEPIIISRDW ENQLYYDIVI ASKDIILDAF LPITFKFAPL DKVTLHRIRI   400
YVTETMEYYC REKKVHRMEP TKKFLLTEQK GPKLPNLPND ANLSKAKNMG   450
NLLQDPKNGD LVNKEYEYQI FIPSRFNNHQ QLHPDTSYEN IKANHWIKIC   500
LRLSRVVDNK RKHYEISIDS PIHVLHRLCS HANTLLPSYD GHPASFPKET   550
DSSISSILES SDDNINLYHN SNIFFPKEVL SSPVLSPNVQ PLDILIPHLP   600
STSLTRNSRQ FNRNSKSHPS DNTIFNSAKL KSNIYQPESL QRELASPQAI   650
PLSPITSPMS NMEVPPPDFD FSSDFISDAA SGTTTTEVSS SESSILPRDP   700
PSYKDTVLHD NNQKRRPNSK HPTPPSLKAS HPNKNSDKNS SETLNKKESM   750
SKIEENKHKR ETTPKKRENR DVKSLSTPQR EESKDSTSTG NQSNEKNRKR   800
VLSLSSSLHS SPNNSGFAHS ALGNLSNESL RSLNRRESVQ DNLPSTIRHD   850
NPFFTDLNQV LIEDELKNHD KNELNRHSTN TSSTPASARS SFDYSGINIS   900
KDKLNMEPLL SKTETLTNKV NEDSFLRPND SYVDLLEPSV DTTIDITAPY   950
ARNSSAWHPL QNDNDNNQFS PLLGSNENFL NAANAQNSAE SDHNNDIFTQ   1000
GSGLTESSKN SDSEERFISR LSSPEKVLIN TLDNESGLQS INESTL       1046