SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Systematic nameYIL108W
DescriptionPutative zinc metalloproteinase YIL108W

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
2        Proteasomal Degradation
3K518        
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[2]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]
[3]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP40483
SGD YIL108W
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
EC number 3.4.24.-

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length696
Molecular weight77414

	---------+----------+----------+----------+----------+
MVGSKDIDLF NLRENEQIVS PCLIVHGKCN KQNGAKTVQV QHPQLPPITY   50
PIHNQFFKAT VILTPGENKL TFVTDTNTAR TIVCYYTPLT QNPPVHLCLI   100
LAKDSPLQFD SPREQKDREG GNGLELAIKK LRLGARLMQA YTNEQMLRNS   150
MGNRTFPFVE EFTWDTLFER PAMRNTIKIH VVRSEKTVKE IQDPDIAQQN   200
SKGKNTGALF GIAMDALKSY GGPFTNNEKP VQAACMFLDT HWDGKLIRGH   250
AALGGGDDSI KLAIFGSHGL YSWPTCLEQL VPYFTDETRS STSEVANDCN   300
ECGTYWECLT ITLGAFMHEI GHLLGCPHQE SGVMLRGYTT LNRSFLTKEA   350
YSVRTNSTGA SPPIFPKEEC TWNRLDTVRF LYHPSFTLPQ DYYDPSFMRP   400
TKLGGYPNIK HSVYPLGNGS CRILSPTGIY LIEIICDDLA RGHIEYLPVS   450
LGGQGPQREV IVTLDDLRAR LPKNELAKFG NTFKLKILSV NAPETEFDKF   500
PSLLDVQPLD MSKYGFSKNV QGIKSPLYGR SDGGNAVGVV AFDVRLVTAV   550
RIYHGYALDG VRFYYKEKPT GTKDAPASKP SVPPRNYFSK ITHSIKNHAS   600
INEENLKSVL FGHETQNFTD ATLEPGEIII GFNLRCGAWV DAIQIITSHG   650
RMTDMFGNKD GGGFAELQPP NGQYILGVTG RVGQWVDAFG IIYGAL       696

	* Ubiquitinated lysine is denoted as red color.