SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Systematic nameYHR097C
DescriptionUncharacterized protein YHR097C

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1K78;K187;K242        
2         
3         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"A proteomics approach to understanding protein ubiquitination."
Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
Nat Biotechnol. 21(8):921-6 (2003) [12872131]
[2]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[3]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP38809
SGD YHR097C
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Cellular componentGO:0005634nucleusIDA:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
7Phosphothreonine.
25Phosphoserine.
69Phosphoserine.
76Phosphothreonine.
189Phosphothreonine.
205Phosphothreonine.
221Phosphoserine.
222Phosphoserine.
223Phosphothreonine.
288Phosphoserine.
294Phosphoserine.
297Phosphoserine.
315Phosphoserine.
359Phosphoserine.

Sequence Information
Sequence length366
Molecular weight40663

	---------+----------+----------+----------+----------+
MNQTGRTIGG PQNGVNTVIN PFRVSPSEDR VSSRDETPRN YNNPFLNEDD   50
TRRAHNSSVS NSRQERLPSY EEAAGTPKQQ APYPKEKKRS SGSNSHQHNH   100
HHHRRTSHGH RDKDKQKSKS RTKVKPPKNV DTIDKMDVTG LFGGSFHHDG   150
PFDACTPQRN KNNKVAPVLA FPADGPNNTV GGRTSKKSTL DEVFGRETVD   200
DDSETLNQLQ DRAYLFNKAN SSTTTLDAIK PNSKNITQFD SKMKTELVHG   250
PITMGLGSTT FLDGAPASSA AIEQDVINHA QESRRKNSIA RKKSLPSRRH   300
LQVNNNNLKL VKTHSGHLEQ KDVDDNRTSV PVTATQGSGH EDVVKKENTG   350
NKLLRRVKSL KTSKKH                                        366

	* Ubiquitinated lysine is denoted as red color.