SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Systematic nameYHL024W
DescriptionUncharacterized RNA-binding protein YHL024W

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI HECTNARSP5   
2   HECTNARSP5   
* Blank: not determined, NA: not applicable.

References
[1]EvidenceUb-conjugate Confirmation
Methodluminescent assay (biotin-ubiquitin, GST-substrate)
Reference"A high throughput screen to identify substrates for the ubiquitin ligase Rsp5."
Kus B, Gajadhar A, Stanger K, Cho R, Sun W, Rouleau N, Lee T, Chan D, Wolting C, Edwards A, Bosse R, Rotin D.
J Biol Chem. 280(33):29470-8 (2005) [15955809]
[2]EvidenceUb-conjugate Confirmation
Methodprotein microarray (in vitro)
Reference"Ubiquitination screen using protein microarrays for comprehensive identification of Rsp5 substrates in yeast."
Gupta R, Kus B, Fladd C, Wasmuth J, Tonikian R, Sidhu S, Krogan NJ, Parkinson J, Rotin D.
Mol Syst Biol. 3:116 (2007) [17551511]

Cross References
Swiss-Prot accessionP38741
SGD YHL024W
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Molecular functionGO:0003723RNA bindingIMP:SGD.
Biological processGO:0007131meiotic recombinationIMP:SGD.
Biological processGO:0006279premeiotic DNA synthesisIMP:SGD.
Biological processGO:0030435sporulationIGI:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length713
Molecular weight80109

	---------+----------+----------+----------+----------+
MKTEISTADS LRDPPSNGLK ADSELVIRED IDQFLPSEVS SLGSDHQNDG   50
EDSDTDSDNF LQDPEDDVDE ESTGRGTVTT TSTSTESRGR PSSCIFVASL   100
AAALSDDELC LSVTENFKKY GDLARVKVLR DNANRPYAFV QYNNDHDAKH   150
ALIRAQGTLL NGRRLRCEPA KVNRTLYLKN QQSIDFNEIS QICEKFGGLE   200
QIVPDRTDNQ YTRRYTYPIS SANSWFVQFV YRDDAIRAYA NLRTDPNWII   250
EWAQNINVPK NYNLLHKSKF KSSKYHQNNG IINNDGSNNN DNNNSNNNNR   300
EDSRRNGDVI EEECGHVHGS DSEEKLTSDG IYDDEDKDSE ITIDKRSIFV   350
GQLDKETTRE ELNRRFSTHG KIQDINLIFK PTNIFAFIKY ETEEAAAAAL   400
ESENHAIFLN KTMHVQYKEV GGRHNRKFSG KNGGSNFNHH QFFSTRSGKT   450
FTGPELNLAP PPINMYRKMS GGSQQESETM MPYMPMGPMP MGPPPPNAAS   500
LSDFDMFPPS YSTFMKGMMP LRRKSMPNSW SSPSSKSVNS ENESVNGGDE   550
NSELPSEIPE SSGRYNAANS FTTYNNSSAG NSNNNNNNNN SNSNKSQYKK   600
RYARRSSYGY NEVPPKPYYF QPYYYHPMQY HMGPMGPLHP SQGSAGNHHP   650
YMMVYPMSPP PPSGLDGSMI PPPINVSQSH AANHGSTHVH ANEFISNDTG   700
DINEDNKAYS LDY                                           713