SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Systematic nameYHL010C
DescriptionRING finger protein YHL010C

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1K369;K410        
2K369;K410        
3         
4         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"A proteomics approach to understanding protein ubiquitination."
Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
Nat Biotechnol. 21(8):921-6 (2003) [12872131]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS
Reference"A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery."
Hitchcock AL, Auld K, Gygi SP, Silver PA.
Proc Natl Acad Sci U S A. 100(22):12735-40 (2003) [14557538]
[3]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[4]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP38748
SGD YHL010C
Gene Ontology (GO)
Molecular functionGO:0008139nuclear localization sequence bindingIDA:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length585
Molecular weight67503

	---------+----------+----------+----------+----------+
MDQFEYIITL EFGNQNQVES AYQIFKSIPK KLKAKSIGEE SIKSNNQDWQ   50
DWRVCDLEID MITDFKNQTS KEEESDLITS QYLGHGIIRL FKLSNANNTL   100
NEKEILTIPG DDTMICILFV PTYFTVHDLL HFYIGDDIVN KQVSNFRILR   150
NQQKGMGFNF TVLIKFRNAL DAKNFKEEFN GKSFSRMDPE TCHVISVKEI   200
VFQKKLFQRP AANEDFPYLL TDPFTVKKKK ELVKVELPTC PVCLERMDSE   250
TTGLVTIPCQ HTFHCQCLNK WKNSRCPVCR HSSLRLSRES LLKQAGDSAH   300
CATCGSTDNL WICLICGNVG CGRYNSKHAI KHYEETLHCF AMDIRTQRVW   350
DYAGDNYVHR LVQNEVDGKL VEVGGSGDDD NNDIGNSDEL QNVVYGNRSK   400
NGEKSNSNKK DGELAANFLR HREYHLEYVQ VLISQLESQR EYYELKLQEK   450
DQTASDSSNV ESLKKSMEDL KLQFQVTQKE WQKREMAQKS KLEEDMLVIE   500
GLQANLDHLS KKQEQLEREN KALEESKQDL EEQVKDLMFY LDSQEKFKDA   550
DESVKEGTIL IQQPHGAAQA SKSKKKRNKN KKAGK                   585

	* Ubiquitinated lysine is denoted as red color.