SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameYHB1
Systematic nameYGR234W
AliasYHB
DescriptionFlavohemoprotein

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP39676
SGD YGR234W
Gene Ontology (GO)
Cellular componentGO:0005829cytosolIDA:SGD.
Cellular componentGO:0005759mitochondrial matrixIDA:SGD.
Molecular functionGO:0016966nitric oxide reductase activityIMP:SGD.
Biological processGO:0006950response to stressIDA:SGD.
EC number 1.14.12.17

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length399
Molecular weight44646

	---------+----------+----------+----------+----------+
MLAEKTRSII KATVPVLEQQ GTVITRTFYK NMLTEHTELL NIFNRTNQKV   50
GAQPNALATT VLAAAKNIDD LSVLMDHVKQ IGHKHRALQI KPEHYPIVGE   100
YLLKAIKEVL GDAATPEIIN AWGEAYQAIA DIFITVEKKM YEEALWPGWK   150
PFDITAKEYV ASDIVEFTVK PKFGSGIELE SLPITPGQYI TVNTHPIRQE   200
NQYDALRHYS LCSASTKNGL RFAVKMEAAR ENFPAGLVSE YLHKDAKVGD   250
EIKLSAPAGD FAINKELIHQ NEVPLVLLSS GVGVTPLLAM LEEQVKCNPN   300
RPIYWIQSSY DEKTQAFKKH VDELLAECAN VDKIIVHTDT EPLINAAFLK   350
EKSPAHADVY TCGSLAFMQA MIGHLKELEH RDDMIHYEPF GPKMSTVQV    399