SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Systematic nameYGR161C
DescriptionUncharacterized protein YGR161C

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI    RPN10 Proteasomal Degradation
2 MULTI    RPN10 Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP53289
SGD YGR161C
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Cellular componentGO:0005634nucleusIDA:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
110Phosphoserine.
152Phosphothreonine.
192Phosphoserine.
216Phosphoserine.
219Phosphoserine.
220Phosphoserine.
238Phosphoserine.

Sequence Information
Sequence length263
Molecular weight29263

	---------+----------+----------+----------+----------+
MIATSRAVNM NKESKHKKAV AKPCRERQTS VTRAMRPAVA RDPRRLSTSS   50
SPSSSPMSAQ RRLSREEIIN EMEKEQDAIV VRLLREIETL KEENSRLKNQ   100
LHHPVPARRS SPFFEGESAI LDDDDCNYGY TLDTPKLKLT DGASRHTVLP   150
LTPKDSMTHI SHSARRSSRN ASISNGTSIS DTIFPIETKI HSAPTTNRNL   200
PSADLPHHTL LPRSLSGISS SDLTESGALL HDRRRRSSNY SLDGSNSLKA   250
DLMAKRFQTG SLK                                           263