SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Systematic nameYGR125W
DescriptionUncharacterized vacuolar membrane protein YGR125W

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP53273
SGD YGR125W
Gene Ontology (GO)
Cellular componentGO:0000324vacuole, cell cycle-correlated morphologyIDA:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
42Phosphoserine.
140Phosphoserine.
149Phosphoserine.
152Phosphoserine.
153Phosphoserine.
842Phosphoserine.
847Phosphothreonine.

Sequence Information
Sequence length1036
Molecular weight116971

	---------+----------+----------+----------+----------+
MGRTIRRRRS NSSLSEAISV SLGINQDSSV NKMHRASVSA MSPPLCRSYM   50
SGFFTGGNSP MINNLSDSKL PISNKQHPKV IHGSENLHRQ TAQLSNEFCS   100
SSVEENSPTI KDYMDIIGNG DRKDDQSMRT IEENIDEEYS DEYSRLLLSP   150
ASSNVDDDRN RGLQNSSLPE LEDGYAGGYQ SLRPSHNLRF RPRNLWHMCT   200
SFPSKFAHYL PAAVLGLLLN ILDALSYGMI IFPITEPVFS HLGPTGISMF   250
YISTIISQAV YSGGWSSFPS GIGSEMIEIT PFYHTMALAI KEALAGNDDE   300
IITTTIFCYV ISSMLTGVVF YALGKLRLGK IVGFFPRHIL IGCIGGVGYF   350
LIITGIEVTT RVAKFEYSWP FFSGLFTDYD TLAKWLLPVL LTVVLIGTQR   400
YFKNSLVLPS FYILTLVLFH FIVAIIPTLS LDALRQAGWI FPIANSDSKW   450
YDHYRLFNVH KVHWSLVLQQ IPTMMALTFF GILHVPINVP ALAMSLQMDK   500
YDVDRELIAH GYSNFFSGLL GSVQNYLVYT NSVLFIRAGA DSPFAGFLLI   550
ALTICIMIIG PVIISFIPIC IVGSLIFLLG YELLVEALVD TWNKLNRFEY   600
LTVVIIVFTM GIFDFVLGII VGILIACFSF LVDSTKLQTI NGEYNGNVAR   650
STVYRDYVQT KFLDGIGEQI YVLKLQNLLF FGTIISIEEK IERLLQISNK   700
DATKRRIKYL ILDFKNINAD NIDYSAAEGF NRIKRFTETK RIKLIISSIK   750
ERDRIYNAFN NVGLLNDVEL FADLNSALEW CENEFLFQYK QLRKKAKERL   800
EEGKQNNVVS AVIAATKNKK IDTIGNGLNR GSNGDTARNL MSLPTNTPRN   850
YQILSVAQNV FVNDEQAVKN FKKEYKDDEP VLPILLFALK QYRPDIISEV   900
QKVREKEIKF WAQLCPYFTR RRLASQSHLL HADNIFFLVE TGMLKATYEL   950
PQGTLYEIFS NGTCFGKIIA PGNAMPREQK LTIETETDSV LWVIDSSSLN   1000
KLKEDNLALY VEVALMVMCI KDTRFKELLG YTLVSA                  1036