SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Systematic nameYGR068C
DescriptionUncharacterized protein YGR068C

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
2   HECTNARSP5   
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[2]EvidenceUb-conjugate Confirmation
Methodprotein microarray (in vitro)
Reference"Ubiquitination screen using protein microarrays for comprehensive identification of Rsp5 substrates in yeast."
Gupta R, Kus B, Fladd C, Wasmuth J, Tonikian R, Sidhu S, Krogan NJ, Parkinson J, Rotin D.
Mol Syst Biol. 3:116 (2007) [17551511]

Cross References
Swiss-Prot accessionP53244
SGD YGR068C
Gene Ontology (GO)
Molecular functionGO:0005515protein bindingIPI:IntAct.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
158Phosphoserine.

Sequence Information
Sequence length586
Molecular weight65477

	---------+----------+----------+----------+----------+
MFSLSSLSSS GGHSEQKERE RISYFDIRIN SPYKDIILIQ GSPLELSSIP   50
LSGNLVISVK NEIVVKKISL RLVGRFKLEF LQVGRYKKNS SSLASLVKEK   100
RKIFECYWDN LLVSSKGDVL VGGENAENQH NSSSGRSTSN QDMDTSGNAI   150
FLSKRSLSSP VFNKIIRRKT HSSHRKILEL PENGVTGTPF EGLRENARSR   200
SSSSNTLNNN SHSYSNRDGS GSSYLFLMKR GNYELPFNTM LPPEVCETIE   250
GLQSGSILYS FEAIIDGRQL WDTDLSVHTS PHGPIGSTST SGNGMRTKNK   300
IIIKKFKYLR ILRTLSMDNL AMQEEISVGN TWRDKLQYET SIPSRAVPIG   350
STTPVKIKIF PFEKNIRLDR IEMALIQYYA MKDSSAQIYD DEIAVMKITH   400
LADFGPLTDK LDVDCPFTIP DNLKQITQDC CLQDNLIRVM HKLQVRILLQ   450
RQVDGEYKNL EIKAQLPMLL FISPHLPMKG RLVLFDKHDG KIHFRPGELV   500
PLFLTTYPAQ GLTPGVELNS TTTAHLALPQ PPPNYHESTN DHLMPALQPL   550
GADSVVLTVP SYEQAQAQAS ASSYVTGSVP AYCDDD                  586