SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameYEH1
Systematic nameYLL012W
DescriptionSterol esterase 1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI    RPN10 Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]

Cross References
Swiss-Prot accessionQ07804
SGD YLL012W
Gene Ontology (GO)
Cellular componentGO:0016021integral to membraneIDA:SGD.
Cellular componentGO:0005811lipid particleIDA:SGD.
Molecular functionGO:0004771sterol esterase activityIDA:SGD.
Biological processGO:0016125sterol metabolic processIMP:SGD.
EC number 3.1.1.13

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length573
Molecular weight66508

	---------+----------+----------+----------+----------+
MGVSAVLKRA RNLLATFIVC CFMAVVLVLA LAHHFINEHR DTRSSSTQIE   50
VDDESKRNVH HDHVLTRTNA YATPYLDLEH DKKNGIVYDH TRTVVRKKNH   100
EVGSSSLHKN LFHKFLTKLI FRFIEKEKVT EGVTQGKFNN SSNEIANHEP   150
VFEKIPVQCD NPLQNLILSE DLTLVADLNY YFNQYNIQIE EFRLETEDGF   200
VIDLWHLIPK YRTTDSDKKK RPPILMLHGL LQSSGSFASN GRKSLAYFLY   250
QSGYDIWLGN NRCGFRPEWN EAKVPTLASR WDWDLREMVK YDLTLLIDTV   300
LAKTQFEKLT LISHSQGTTQ GFMGLVNEDK FFPPGSGSKE SFFTSKIANY   350
IALAPAVYPG PLLNEKLFVK LMTKEIENPW FFGETSFFEI MMIVRNLCVG   400
ESLFSFVCYT IFNYLFDWND TLWDTALRDR HFLFSPVHVS VKLMQWWLSP   450
DPNKVSFKFG SHKMFPDNVK WFSDASKAPN IYLFVPKQDR LVDGERLINH   500
FVNVESNVNY KIWYIDEYAH IDVLWAHDVI ERIGKPILQN LNNYYSKKPS   550
SAFESDCSDT EVETELEMVA EKA                                573