SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameYDJ1
Systematic nameYNL064C
AliasMAS5
DescriptionMitochondrial protein import protein MAS5

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI       
2         
3         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[3]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP25491
SGD YNL064C
Gene Ontology (GO)
Cellular componentGO:0005829cytosolIDA:SGD.
Cellular componentGO:0005792microsomeIDA:SGD.
Cellular componentGO:0048471perinuclear region of cytoplasmIDA:SGD.
Molecular functionGO:0001671ATPase activator activityIDA:SGD.
Molecular functionGO:0030192Hsp70/Hsc70 protein regulator activityIDA:SGD.
Molecular functionGO:0051082unfolded protein bindingIDA:SGD.
Biological processGO:0006458'de novo' protein foldingIMP:SGD.
Biological processGO:0030433ER-associated protein catabolic processIMP:SGD.
Biological processGO:0042026protein refoldingIDA:SGD.
Biological processGO:0045047protein targeting to ERIMP:SGD.
Biological processGO:0006626protein targeting to mitochondrionIMP:SGD.
Biological processGO:0042493response to drugIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length409
Molecular weight44671

	---------+----------+----------+----------+----------+
MVKETKFYDI LGVPVTATDV EIKKAYRKCA LKYHPDKNPS EEAAEKFKEA   50
SAAYEILSDP EKRDIYDQFG EDGLSGAGGA GGFPGGGFGF GDDIFSQFFG   100
AGGAQRPRGP QRGKDIKHEI SASLEELYKG RTAKLALNKQ ILCKECEGRG   150
GKKGAVKKCT SCNGQGIKFV TRQMGPMIQR FQTECDVCHG TGDIIDPKDR   200
CKSCNGKKVE NERKILEVHV EPGMKDGQRI VFKGEADQAP DVIPGDVVFI   250
VSERPHKSFK RDGDDLVYEA EIDLLTAIAG GEFALEHVSG DWLKVGIVPG   300
EVIAPGMRKV IEGKGMPIPK YGGYGNLIIK FTIKFPENHF TSEENLKKLE   350
EILPPRIVPA IPKKATVDEC VLADFDPAKY NRTRASRGGA NYDSDEEEQG   400
GEGVQCASQ                                                409