SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Systematic nameYCL063W
DescriptionUncharacterized protein YCL063W

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI    RPN10 Proteasomal Degradation
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]
[2]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP25591
SGD YCL063W
Gene Ontology (GO)
Cellular componentGO:0005774vacuolar membraneIDA:SGD.
Molecular functionGO:0043495protein anchorIPI:SGD.
Biological processGO:0000011vacuole inheritanceIEP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length423
Molecular weight47843

	---------+----------+----------+----------+----------+
MATQALEDIT ERLLIRSQEA ILQLDLWIQR QQRSSICQTT DQESLDKLSQ   50
QYNQYMSQLN SLYVRSESVR DKLSKEQQRR LITEDNEHQR IEDLVREFQD   100
ITLRLNELAT VPNEAPNDSP QSQSTRSSLG SFQPRPLKII ERQRLCMVTP   150
SKPPKKSVGF NPINEVDCPS KTNSLPCSPK KQPARNRTLR AAKSHDTGLN   200
KSKKPSSSDT YESFFKNRQR LSLTFFDEMD DEDFDSDQDT IILPNISTPP   250
HVGVTAKGAE FEPLRRYNSH ESILSNKPAP SKSLNLGSFS ASFFRPSNPT   300
FGTSISNVQV NCHPTVAATM APSRNGPRIS SSKALLSSFI ARSDTHTVKE   350
NNTNLKHASF MDKFNSSLST ISESFQSKRG RKNKGMNEER ISNHNVAQEQ   400
KNNMDISVSI EELQDALNTE LLF                                423