SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameYCF1
Systematic nameYDR135C
DescriptionMetal resistance protein YCF1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP39109
SGD YDR135C
Gene Ontology (GO)
Cellular componentGO:0005774vacuolar membraneIDA:SGD.
Molecular functionGO:0015127bilirubin transporter activityIGI:SGD.
Molecular functionGO:0015431glutathione S-conjugate-exporting ATPase ac...IDA:SGD.
Biological processGO:0015723bilirubin transportIGI:SGD.
Biological processGO:0042493response to drugIMP:SGD.
Biological processGO:0010038response to metal ionIDA:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
251Phosphoserine.
878Phosphoserine.
903Phosphoserine.
908Phosphoserine.
911Phosphothreonine.
914Phosphoserine.

Sequence Information
Sequence length1515
Molecular weight171121

	---------+----------+----------+----------+----------+
MAGNLVSWAC KLCRSPEGFG PISFYGDFTQ CFIDGVILNL SAIFMITFGI   50
RDLVNLCKKK HSGIKYRRNW IIVSRMALVL LEIAFVSLAS LNISKEEAEN   100
FTIVSQYAST MLSLFVALAL HWIEYDRSVV ANTVLLFYWL FETFGNFAKL   150
INILIRHTYE GIWYSGQTGF ILTLFQVITC ASILLLEALP KKPLMPHQHI   200
HQTLTRRKPN PYDSANIFSR ITFSWMSGLM KTGYEKYLVE ADLYKLPRNF   250
SSEELSQKLE KNWENELKQK SNPSLSWAIC RTFGSKMLLA AFFKAIHDVL   300
AFTQPQLLRI LIKFVTDYNS ERQDDHSSLQ GFENNHPQKL PIVRGFLIAF   350
AMFLVGFTQT SVLHQYFLNV FNTGMYIKSA LTALIYQKSL VLSNEASGLS   400
STGDIVNLMS VDVQKLQDLT QWLNLIWSGP FQIIICLYSL YKLLGNSMWV   450
GVIILVIMMP LNSFLMRIQK KLQKSQMKYK DERTRVISEI LNNIKSLKLY   500
AWEKPYREKL EEVRNNKELK NLTKLGCYMA VTSFQFNIVP FLVSCCTFAV   550
FVYTEDRALT TDLVFPALTL FNLLSFPLMI IPMVLNSFIE ASVSIGRLFT   600
FFTNEELQPD SVQRLPKVKN IGDVAINIGD DATFLWQRKP EYKVALKNIN   650
FQAKKGNLTC IVGKVGSGKT ALLSCMLGDL FRVKGFATVH GSVAYVSQVP   700
WIMNGTVKEN ILFGHRYDAE FYEKTIKACA LTIDLAILMD GDKTLVGEKG   750
ISLSGGQKAR LSLARAVYAR ADTYLLDDPL AAVDEHVARH LIEHVLGPNG   800
LLHTKTKVLA TNKVSALSIA DSIALLDNGE ITQQGTYDEI TKDADSPLWK   850
LLNNYGKKNN GKSNEFGDSS ESSVRESSIP VEGELEQLQK LNDLDFGNSD   900
AISLRRASDA TLGSIDFGDD ENIAKREHRE QGKVKWNIYL EYAKACNPKS   950
VCVFILFIVI SMFLSVMGNV WLKHWSEVNS RYGSNPNAAR YLAIYFALGI   1000
GSALATLIQT IVLWVFCTIH ASKYLHNLMT NSVLRAPMTF FETTPIGRIL   1050
NRFSNDIYKV DALLGRTFSQ FFVNAVKVTF TITVICATTW QFIFIIIPLS   1100
VFYIYYQQYY LRTSRELRRL DSITRSPIYS HFQETLGGLA TVRGYSQQKR   1150
FSHINQCRID NNMSAFYPSI NANRWLAYRL ELIGSIIILG AATLSVFRLK   1200
QGTLTAGMVG LSLSYALQIT QTLNWIVRMT VEVETNIVSV ERIKEYADLK   1250
SEAPLIVEGH RPPKEWPSQG DIKFNNYSTR YRPELDLVLK HINIHIKPNE   1300
KVGIVGRTGA GKSSLTLALF RMIEASEGNI VIDNIAINEI GLYDLRHKLS   1350
IIPQDSQVFE GTVRENIDPI NQYTDEAIWR ALELSHLKEH VLSMSNDGLD   1400
AQLTEGGGNL SVGQRQLLCL ARAMLVPSKI LVLDEATAAV DVETDKVVQE   1450
TIRTAFKDRT ILTIAHRLNT IMDSDRIIVL DNGKVAEFDS PGQLLSDNKS   1500
LFYSLCMEAG LVNEN                                         1515