SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Systematic nameYBR025C
DescriptionUncharacterized GTP-binding protein YBR025C

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP38219
SGD YBR025C
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Molecular functionGO:0016887ATPase activityIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0006200ATP catabolic processIDA:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
67Phosphoserine.

Sequence Information
Sequence length394
Molecular weight44174

	---------+----------+----------+----------+----------+
MPPKKQVEEK KVLLGRPGNN LKAGIVGLAN VGKSTFFQAI TRCPLGNPAN   50
YPFATIDPEE ARVIVPSPRF DKLCEIYKKT ASEVPAHLTV YDIAGLTKGA   100
SAGEGLGNAF LSHIRSVDSI YQVVRCFDDA EIIHVEGDVD PVRDLEIINQ   150
ELRLKDIEFA QKALEGAEKI AKRGGQSLEV KQKKEEMDLI TKIIKLLESG   200
QRVANHSWTS KEVEIINSMF LLTAKPCIYL INLSERDYIR KKNKHLLRIK   250
EWVDKYSPGD LIIPFSVSLE ERLSHMSPED AEEELKKLQT ISALPKIITT   300
MRQKLDLISF FTCGPDEVRE WTIRRGTKAP QAAGVIHNDL MNTFILAQVM   350
KCEDVFEYKD DSAIKAAGKL MQKGKDYVVE DGDIIYFRAG AGKN         394