SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameVTS1
Systematic nameYOR359W
DescriptionProtein VTS1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI    RPN10 Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]

Cross References
Swiss-Prot accessionQ08831
SGD YOR359W
Gene Ontology (GO)
Cellular componentGO:0005829cytosolIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Molecular functionGO:0003723RNA bindingIDA:SGD.
Biological processGO:0006623protein targeting to vacuoleIGI:SGD.
Biological processGO:0043488regulation of mRNA stabilityIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length523
Molecular weight57557

	---------+----------+----------+----------+----------+
MKHPYEEFPT GSKSPYNMSR GAHPGAVLLS PQSSAINKNN PGSNSGNNQG   50
NSSVTANVLS PQSHSMSLND MLDQQSFMLD TAGTRAQPLQ QQQQQQQQQQ   100
QASLPSLNIQ TVSSTAAGSA IVSPMMQSPK ALQSTLSSTS MYLDSFQRSP   150
NNILGIPSQS GSIPLPQSRQ SQQQSQSQKN DPNMGTNFSQ DINQLCSWIS   200
MLNSSQQNTV MDNILSILND DVLKYTKLKI ETLTNTPFIS PPLPAIASPI   250
PNRDDTQILN IDSVFSSSPI TNDPENTDNL LYQNWSPQPH SIPISQPIYD   300
NITDASQRSK SAEPHVNSSP NLIPVQKQFN NGNSTKYKKL PSENPNYLSH   350
SLSSSHSFFQ PKKRSNMGNE YNSHHHHSLH HPLHNTTSYF SNTSRPSGTD   400
LNKSNQNVFN NTITHPNAGP TSATSTSTSS NGNTPLSSNS SMNPKSLTDP   450
KLLKNIPMWL KSLRLHKYSD ALSGTPWIEL IYLDDETLEK KGVLALGARR   500
KLLKAFGIVI DYKERDLIDR SAY                                523