SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


Main Search Enzymes Substrates Ubiquitination types BLAST Feedback HELP

Substrate Information

General Information
Standard nameVTC4
Systematic nameYJL012C
AliasPHM3
DescriptionVacuolar transporter chaperone 4

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI       
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]

Cross References
Swiss-Prot accessionP47075
SGD YJL012C
Gene Ontology (GO)
Cellular componentGO:0031310intrinsic to vacuolar membraneIDA:SGD.
Biological processGO:0042144vacuole fusion, non-autophagicIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length721
Molecular weight83155

	---------+----------+----------+----------+----------+
MKFGEHLSKS LIRQYSYYYI SYDDLKTELE DNLSKNNGQW TQELETDFLE   50
SLEIELDKVY TFCKVKHSEV FRRVKEVQEQ VQHTVRLLDS NNPPTQLDFE   100
ILEEELSDII ADVHDLAKFS RLNYTGFQKI IKKHDKKTGF ILKPVFQVRL   150
DSKPFFKENY DELVVKISQL YDIARTSGRP IKGDSSAGGK QQNFVRQTTK   200
YWVHPDNITE LKLIILKHLP VLVFNTNKEF EREDSAITSI YFDNENLDLY   250
YGRLRKDEGA EAHRLRWYGG MSTDTIFVER KTHREDWTGE KSVKARFALK   300
ERHVNDFLKG KYTVDQVFAK MRKEGKKPMN EIENLEALAS EIQYVMLKKK   350
LRPVVRSFYN RTAFQLPGDA RVRISLDTEL TMVREDNFDG VDRTHKNWRR   400
TDIGVDWPFK QLDDKDICRF PYAVLEVKLQ TQLGQEPPEW VRELVGSHLV   450
EPVPKFSKFI HGVATLLNDK VDSIPFWLPQ MDVDIRKPPL PTNIEITRPG   500
RSDNEDNDFD EDDEDDAALV AAMTNAPGNS LDIEESVGYG ATSAPTSNTN   550
HVVESANAAY YQRKIRNAEN PISKKYYEIV AFFDHYFNGD QISKIPKGTT   600
FDTQIRAPPG KTICVPVRVE PKVYFATERT YLSWLSISIL LGGVSTTLLT   650
YGSPTAMIGS IGFFITSLAV LIRTVMVYAK RVVNIRLKRA VDYEDKIGPG   700
MVSVFLILSI LFSFFCNLVA K                                  721