SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameVPS9
Systematic nameYML097C
AliasVPT9
DescriptionVacuolar protein sorting-associated protein 9

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MONO HECTNARSP5   
2 MONO HECTNARSP5   
* Blank: not determined, NA: not applicable.

References
[1]EvidenceHigh MW Product Confirmation
MethodWestern blot
Reference"A ubiquitin-binding motif required for intramolecular monoubiquitylation, the CUE domain."
Shih SC, Prag G, Francis SA, Sutanto MA, Hurley JH, Hicke L.
EMBO J. 22(6):1273-81 (2003) [12628920]
[2]EvidenceUb-conjugate Confirmation
MethodWestern blot
Reference"Vps9p CUE domain ubiquitin binding is required for efficient endocytic protein traffic."
Davies BA, Topp JD, Sfeir AJ, Katzmann DJ, Carney DS, Tall GG, Friedberg AS, Deng L, Chen Z, Horazdovsky BF.
J Biol Chem. 278(22):19826-33 (2003) [12654912]

Cross References
Swiss-Prot accessionP54787
SGD YML097C
Gene Ontology (GO)
Cellular componentGO:0005829cytosolIDA:SGD.
Molecular functionGO:0005085guanyl-nucleotide exchange factor activityIDA:SGD.
Molecular functionGO:0042802identical protein bindingIPI:IntAct.
Biological processGO:0006623protein targeting to vacuoleIMP:SGD.
Biological processGO:0000723telomere maintenanceIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length451
Molecular weight52394

	---------+----------+----------+----------+----------+
MTDDEKREIL KEFDPFSQLE QANGNPDKDV KFKKDDPNRA AAEETNRDIS   50
AQDKGDEEPF YDFQIFIKQL QTPGADPLVK YTKSFLRNFL AQRLLWTVSE   100
EIKLISDFKT FIYDKFTLYE PFRSLDNSKM RNAKEGMEKL IMGKLYSRCF   150
SPSLYEILQK PLDDEHMKDL TNDDTLLEKI RHYRFISPIM LDIPDTMPNA   200
RLNKFVHLAS KELGKINRFK SPRDKMVCVL NASKVIFGLL KHTKLEQNGA   250
DSFIPVLIYC ILKGQVRYLV SNVNYIERFR SPDFIRGEEE YYLSSLQAAL   300
NFIMSLTERS LTIDDHEDFE EAYQRNLKQL AEEKEEEEKK KQLEVPDELQ   350
PNGTLLKPLD EVTNIVISKF NELFSPIGEP TQEEALKSEQ SNKEEDVSSL   400
IKKIEENERK DTLNTLQNMF PDMDPSLIED VCIAKKSRIG PCVDALLSLS   450
E                                                        451