SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameVPS74
Systematic nameYDR372C
DescriptionVacuolar protein sorting-associated protein 74

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionQ06385
SGD YDR372C
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Cellular componentGO:0005634nucleusIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0006623protein targeting to vacuoleIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
14Phosphoserine.
17Phosphothreonine.
19Phosphoserine.
27Phosphothreonine.

Sequence Information
Sequence length345
Molecular weight39287

	---------+----------+----------+----------+----------+
MSTLQRRRVN RADSGDTSSI HSSANNTKGD KIANIAVDGD DDNGTNKKIA   50
YDPEESKLRD NINIPTLTLM EEVLLMGLRD REGYLSFWND SISYALRGCI   100
IIELALRGKI RILDDSARKR FDLSERLIEV IDSSKTGEVL LDETLQLMKN   150
DEPLSISNWI DLLSGETWNL LKINYQLKQV RERLAKGLVD KGVLRTEMKN   200
FFLFDMATHP IADASCKEAI KRRVLSVLVS RNMELSYNEY FPETTSFKII   250
RTLALICGSY GANVLENVLT TLEYEKRDKA ISRAEEIMAQ FSQYPFDLEK   300
ETELGVSVNL NKEVKEEIEN NPGHDLQLEV IAGVFEVFSR MDMLL        345