SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameVPS72
Systematic nameYDR485C
AliasSWC2
DescriptionVacuolar protein sorting-associated protein 72

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI    RPN10 Proteasomal Degradation
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionQ03388
SGD YDR485C
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Cellular componentGO:0000812SWR1 complexIDA:SGD.
Molecular functionGO:0042393histone bindingIPI:SGD.
Biological processGO:0043486histone exchangeIMP:SGD.
Biological processGO:0006623protein targeting to vacuoleIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
396Phosphoserine.

Sequence Information
Sequence length795
Molecular weight90580

	---------+----------+----------+----------+----------+
MSDEGADKSL DTNTEFIIQT RSRRSNAGNK LQKLLEQELR DIESTKRQIS   50
SYKNGNDDEE DEIGLLFQED EDDEDFEMMA KDDDDEGEEK EDETQSIRKE   100
PSQASSEQAA DDLMFSSSES EDSSNENDED AEEKEIRRQE LLSRKKRNKR   150
LQKGPVVIKK QKPKPKSGEA IPRSHHTHEQ LNAETLLLNT RRTSKRSSVM   200
ENTMKVYEKL SKAEKKRKII QERIRKHKEQ ESQHMLTQEE RLRIAKETEK   250
LNILSLDKFK EQEVWKKENR LALQKRQKQK FQPNETILQF LSTAWLMTPA   300
MELEDRKYWQ EQLNKRDKKK KKYPRKPKKN LNLGKQDASD DKKRESEESI   350
KNDGDVNSLG ENSSSVHNQK RIEETSTNDT VEGESSPDAA VSRVNSDELK   400
PTALPDVTLD AIANKQSTVD EAPNSQPQKN IITNEQKITN VGEPIQNLHN   450
EEIKDEMVSA LESRENTFEN SSPAAQVVSQ RDNSATPTPS NSTGTEDTIL   500
ISPDTDIKGE PEPCLKTEGI ENLSHNVPQE TKSNTDVSFL KQVTFTDHPQ   550
VAIIDTEESP SKKDTANVDE SSAENSLSTQ TYEGPEQLTS RNFVTLYDFP   600
NAPPNLKDFN TNLFGDRWSY TNGLSATQRP QDMKTVFHSI LPSPPQSSVP   650
SPTVDISLDL SALANFPSFG EYDKKIVHQI NTEINKDLEI KIKTQPPTGV   700
FLANGIRKKC LITNKECQYF DPRTGVPYSD VEAYKIIQRI QDPISKEEGR   750
SDIKRDETTN EDSDDQVRFK WFGFKNGGIY LDLSQRPAKG VPEGF        795