SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameVPS54
Systematic nameYDR027C
AliasCGP1, LUV1, RKI1, TCS3
DescriptionVacuolar protein sorting-associated protein 54

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionQ12071
SGD YDR027C
Gene Ontology (GO)
Cellular componentGO:0000938GARP complexIPI:SGD.
Cellular componentGO:0005739mitochondrionIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0006896Golgi to vacuole transportIMP:SGD.
Biological processGO:0042147retrograde transport, endosome to GolgiIDA:SGD.
Biological processGO:0000723telomere maintenanceIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
29Phosphoserine.
69Phosphoserine.
72Phosphoserine.

Sequence Information
Sequence length889
Molecular weight101520

	---------+----------+----------+----------+----------+
MSISETPHNK SQGLQKAAGR PKIVVPEGSP SRNSDSGSFT IEGDTSLNDD   50
LLSISGSVTP RARRSSRLSL DSITPRRSFD SRTLSVANSR SFGFENETHS   100
GSMDFSPLGN NSIYEIVMNT RRKNWLNYPT VADIPQVSLS KNDLDDHWKT   150
HVIEYVKNIK SDYQIFQSTN NIRNMNQMEQ LKELREGENM HEESFEANLR   200
QGDAELINSI PDFYFSDKFQ LDNPRTFHKV LDAIDLFLTK LDMKRQAERD   250
EAFSELRDRL NDFLDIVETL LVTEISKSSH KFFHALSEVD NIQKRALDTM   300
SELKELAQNI KTIDAENIRK KISHLEMIFK RKNVEKLEQG LLQAKLVLNK   350
TDECKSMYEE NKLDNCLELI KSIDYLIKGD DSINEDVQSW TRCWPYKLSN   400
LRTIPALSAT REFLTNMKIE IGGKFSLQLS ILLIDDLRSF CKSIKPKETL   450
HRIQTGSNDK KQTIFTDNFS SKITELIVRL NRCEELTSAF DLYREKSITE   500
LKSIIKIYLP TENAHADNNH DEKHLNNGST SGSKLSRLIK EQTPAEFQSM   550
LVNIFTHALE ALRRLYGHQK LLLDISLNEL ASVKSPNENQ HNMITQLDIR   600
TGINEIIRII QLRTGKIIAV RRELNLSLRY DYFLKFYAIC VIFIQECEVL   650
SGEFLTKYLS NVLASQIKHY ANAQSSKNYR NIKKKIDAEE WIPYIVDSSI   700
QSDVNDIVSS IDIDPLSWTT ILDMVGGSHD CENGRSEDKE KDEGNETYQG   750
HRKSVVVGDK TFVASSSLLA TIEVIKELMV LSINLPSIYL SNFEKLCYDA   800
LQYYNSSAMA SVTQPGNSLL KTGRNLSIMG ESLDCLAEFV IIVQRFYQRL   850
SNSNRDFEPF DASHYTTLLG QFQASSNKIY MANAPPPPV               889