SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameVPS4
Systematic nameYPR173C
AliasCSC1, DID6, END13, GRD13, VPL4, VPT10
DescriptionVacuolar protein sorting-associated protein 4

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP52917
SGD YPR173C
Gene Ontology (GO)
Cellular componentGO:0005783endoplasmic reticulumIDA:SGD.
Molecular functionGO:0016887ATPase activityIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0045324late endosome to vacuole transportIMP:SGD.
Biological processGO:0045053protein retention in GolgiIMP:SGD.
Biological processGO:0016125sterol metabolic processIGI:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
120Phosphoserine.

Sequence Information
Sequence length437
Molecular weight48172

	---------+----------+----------+----------+----------+
MSTGDFLTKG IELVQKAIDL DTATQYEEAY TAYYNGLDYL MLALKYEKNP   50
KSKDLIRAKF TEYLNRAEQL KKHLESEEAN AAKKSPSAGS GSNGGNKKIS   100
QEEGEDNGGE DNKKLRGALS SAILSEKPNV KWEDVAGLEG AKEALKEAVI   150
LPVKFPHLFK GNRKPTSGIL LYGPPGTGKS YLAKAVATEA NSTFFSVSSS   200
DLVSKWMGES EKLVKQLFAM ARENKPSIIF IDEVDALTGT RGEGESEASR   250
RIKTELLVQM NGVGNDSQGV LVLGATNIPW QLDSAIRRRF ERRIYIPLPD   300
LAARTTMFEI NVGDTPCVLT KEDYRTLGAM TEGYSGSDIA VVVKDALMQP   350
IRKIQSATHF KDVSTEDDET RKLTPCSPGD DGAIEMSWTD IEADELKEPD   400
LTIKDFLKAI KSTRPTVNED DLLKQEQFTR DFGQEGN                 437