SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameVPS29
Systematic nameYHR012W
AliasPEP11
DescriptionVacuolar protein sorting-associated protein 29

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP38759
SGD YHR012W
Gene Ontology (GO)
Cellular componentGO:0005768endosomeIPI:SGD.
Cellular componentGO:0030904retromer complexIPI:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0042147retrograde transport, endosome to GolgiIPI:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length282
Molecular weight31013

	---------+----------+----------+----------+----------+
MLLLALSDAH IPDRATDLPV KFKKLLSVPD KISQVALLGN STKSYDFLKF   50
VNQISNNITI VRGEFDNGHL PSTKKDKASD NSRPMEEIPM NSIIRQGALK   100
IGCCSGYTVV PKNDPLSLLA LARQLDVDIL LWGGTHNVEA YTLEGKFFVN   150
PGSCTGAFNT DWPIVFDVED SDEAVTSEVD KPTKENQSED DDAKGGSTGK   200
EQPGSYTPKE GTAGERENEN ESNVKPENQF KEDEVDMSDS DINGSNSPSF   250
CLLDIQGNTC TLYIYLYVNG EVKVDKVVYE KE                      282