SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameVMA7
Systematic nameYGR020C
DescriptionVacuolar proton pump subunit F

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI       
2         
3         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[3]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP39111
SGD YGR020C
Gene Ontology (GO)
Cellular componentGO:0000329membrane of vacuole with cell cycle-correla...TAS:SGD.
Cellular componentGO:0000221vacuolar proton-transporting V-type ATPase,...TAS:SGD.
Molecular functionGO:0046961hydrogen ion transporting ATPase activity, ...TAS:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0007035vacuolar acidificationTAS:SGD.
EC number 3.6.3.14

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length118
Molecular weight13461

	---------+----------+----------+----------+----------+
MAEKRTLIAV IADEDTTTGL LLAGIGQITP ETQEKNFFVY QEGKTTKEEI   50
TDKFNHFTEE RDDIAILLIN QHIAENIRAR VDSFTNAFPA ILEIPSKDHP   100
YDPEKDSVLK RVRKLFGE                                      118