SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameVMA5
Systematic nameYKL080W
AliasVAT3, VATC
DescriptionVacuolar proton pump subunit C

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP31412
SGD YKL080W
Gene Ontology (GO)
Cellular componentGO:0000329membrane of vacuole with cell cycle-correla...TAS:SGD.
Cellular componentGO:0000221vacuolar proton-transporting V-type ATPase,...TAS:SGD.
Molecular functionGO:0046961hydrogen ion transporting ATPase activity, ...TAS:SGD.
Biological processGO:0007035vacuolar acidificationTAS:SGD.
EC number 3.6.3.14

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
2N-acetylalanine.

Sequence Information
Sequence length392
Molecular weight44189

	---------+----------+----------+----------+----------+
MATALYTAND FILISLPQNA QPVTAPGSKT DSWFNETLIG GRAFVSDFKI   50
PEFKIGSLDT LIVESEELSK VDNQIGASIG KIIEILQGLN ETSTNAYRTL   100
PINNMPVPEY LENFQWQTRK FKLDKSIKDL ITLISNESSQ LDADVRATYA   150
NYNSAKTNLA AAERKKTGDL SVRSLHDIVK PEDFVLNSEH LTTVLVAVPK   200
SLKSDFEKSY ETLSKNVVPA SASVIAEDAE YVLFNVHLFK KNVQEFTTAA   250
REKKFIPREF NYSEELIDQL KKEHDSAASL EQSLRVQLVR LAKTAYVDVF   300
INWFHIKALR VYVESVLRYG LPPHFNIKII AVPPKNLSKC KSELIDAFGF   350
LGGNAFMKDK KGKINKQDTS LHQYASLVDT EYEPFVMYII NL           392