SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameVMA2
Systematic nameYBR127C
AliasVAT2
DescriptionVacuolar ATP synthase subunit B

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI       
2         
3         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[3]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP16140
SGD YBR127C
Gene Ontology (GO)
Cellular componentGO:0000221vacuolar proton-transporting V-type ATPase,...IDA:SGD.
Molecular functionGO:0046961hydrogen ion transporting ATPase activity, ...IMP:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0006874calcium ion homeostasisIMP:SGD.
Biological processGO:0007035vacuolar acidificationIMP:SGD.
EC number 3.6.3.14

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
4Phosphoserine.
501Phosphothreonine.
503Phosphoserine.
504Phosphoserine.
511Phosphoserine, by ATM or ATR.
515Phosphoserine.

Sequence Information
Sequence length517
Molecular weight57749

	---------+----------+----------+----------+----------+
MVLSDKELFA INKKAVEQGF NVKPRLNYNT VSGVNGPLVI LEKVKFPRYN   50
EIVNLTLPDG TVRQGQVLEI RGDRAIVQVF EGTSGIDVKK TTVEFTGESL   100
RIPVSEDMLG RIFDGSGRPI DNGPKVFAED YLDINGSPIN PYARIYPEEM   150
ISTGVSAIDT MNSIARGQKI PIFSASGLPH NEIAAQICRQ AGLVRPTKDV   200
HDGHEENFSI VFAAMGVNLE TARFFKQDFE ENGSLERTSL FLNLANDPTI   250
ERIITPRLAL TTAEYLAYQT ERHVLTILTD MSSYADALRE VSAAREEVPG   300
RRGYPGYMYT DLSTIYERAG RVEGRNGSIT QIPILTMPND DITHPIPDLT   350
GYITEGQIFV DRQLHNKGIY PPINVLPSLS RLMKSAIGEG MTRKDHGDVS   400
NQLYAKYAIG KDAAAMKAVV GEEALSIEDK LSLEFLEKFE KTFITQGAYE   450
DRTVFESLDQ AWSLLRIYPK EMLNRISPKI LDEFYDRARD DADEDEEDPD   500
TRSSGKKKDA SQEESLI                                       517