SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameVMA13
Systematic nameYPR036W
AliasCLS11
DescriptionVacuolar proton pump subunit H

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP41807
SGD YPR036W
Gene Ontology (GO)
Cellular componentGO:0000329membrane of vacuole with cell cycle-correla...TAS:SGD.
Cellular componentGO:0000221vacuolar proton-transporting V-type ATPase,...TAS:SGD.
Molecular functionGO:0046961hydrogen ion transporting ATPase activity, ...TAS:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0007035vacuolar acidificationTAS:SGD.
EC number 3.6.3.14

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length478
Molecular weight54416

	---------+----------+----------+----------+----------+
MGATKILMDS THFNEIRSII RSRSVAWDAL ARSEELSEID ASTAKALESI   50
LVKKNIGDGL SSSNNAHSGF KVNGKTLIPL IHLLSTSDNE DCKKSVQNLI   100
AELLSSDKYG DDTVKFFQED PKQLEQLFDV SLKGDFQTVL ISGFNVVSLL   150
VQNGLHNVKL VEKLLKNNNL INILQNIEQM DTCYVCIRLL QELAVIPEYR   200
DVIWLHEKKF MPTLFKILQR ATDSQLATRI VATNSNHLGI QLQYHSLLLI   250
WLLTFNPVFA NELVQKYLSD FLDLLKLVKI TIKEKVSRLC ISIILQCCST   300
RVKQHKKVIK QLLLLGNALP TVQSLSERKY SDEELRQDIS NLKEILENEY   350
QELTSFDEYV AELDSKLLCW SPPHVDNGFW SDNIDEFKKD NYKIFRQLIE   400
LLQAKVRNGD VNAKQEKIII QVALNDITHV VELLPESIDV LDKTGGKADI   450
MELLNHSDSR VKYEALKATQ AIIGYTFK                           478