SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameVMA1
Systematic nameYDL185W
AliasCLS8, TFP1
DescriptionEndonuclease PI-SceI

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP17255
SGD YDL185W
Gene Ontology (GO)
Cellular componentGO:0000329membrane of vacuole with cell cycle-correla...IDA:SGD.
Cellular componentGO:0000221vacuolar proton-transporting V-type ATPase,...TAS:SGD.
Molecular functionGO:0004520endodeoxyribonuclease activityTAS:SGD.
Molecular functionGO:0046961hydrogen ion transporting ATPase activity, ...TAS:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0006314intron homingTAS:SGD.
Biological processGO:0019538protein metabolic processIDA:SGD.
Biological processGO:0007035vacuolar acidificationTAS:SGD.
EC number 3.6.3.14,3.1.-.-

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
2N-acetylalanine.
131Phosphothreonine.
166Phosphoserine.
376Phosphoserine.

Sequence Information
Sequence length1071
Molecular weight118637

	---------+----------+----------+----------+----------+
MAGAIENARK EIKRISLEDH AESEYGAIYS VSGPVVIAEN MIGCAMYELV   50
KVGHDNLVGE VIRIDGDKAT IQVYEETAGL TVGDPVLRTG KPLSVELGPG   100
LMETIYDGIQ RPLKAIKEES QSIYIPRGID TPALDRTIKW QFTPGKFQVG   150
DHISGGDIYG SVFENSLISS HKILLPPRSR GTITWIAPAG EYTLDEKILE   200
VEFDGKKSDF TLYHTWPVRV PRPVTEKLSA DYPLLTGQRV LDALFPCVQG   250
GTTCIPGAFG CGKTVISQSL SKYSNSDAII YVGCFAKGTN VLMADGSIEC   300
IENIEVGNKV MGKDGRPREV IKLPRGRETM YSVVQKSQHR AHKSDSSREV   350
PELLKFTCNA THELVVRTPR SVRRLSRTIK GVEYFEVITF EMGQKKAPDG   400
RIVELVKEVS KSYPISEGPE RANELVESYR KASNKAYFEW TIEARDLSLL   450
GSHVRKATYQ TYAPILYEND HFFDYMQKSK FHLTIEGPKV LAYLLGLWIG   500
DGLSDRATFS VDSRDTSLME RVTEYAEKLN LCAEYKDRKE PQVAKTVNLY   550
SKVVRGNGIR NNLNTENPLW DAIVGLGFLK DGVKNIPSFL STDNIGTRET   600
FLAGLIDSDG YVTDEHGIKA TIKTIHTSVR DGLVSLARSL GLVVSVNAEP   650
AKVDMNGTKH KISYAIYMSG GDVLLNVLSK CAGSKKFRPA PAAAFARECR   700
GFYFELQELK EDDYYGITLS DDSDHQFLLA NQVVVHNCGE RGNEMAEVLM   750
EFPELYTEMS GTKEPIMKRT TLVANTSNMP VAAREASIYT GITLAEYFRD   800
QGKNVSMIAD SSSRWAEALR EISGRLGEMP ADQGFPAYLG AKLASFYERA   850
GKAVALGSPD RTGSVSIVAA VSPAGGDFSD PVTTATLGIT QVFWGLDKKL   900
AQRKHFPSIN TSVSYSKYTN VLNKFYDSNY PEFPVLRDRM KEILSNAEEL   950
EQVVQLVGKS ALSDSDKITL DVATLIKEDF LQQNGYSTYD AFCPIWKTFD   1000
MMRAFISYHD EAQKAVANGA NWSKLADSTG DVKHAVSSSK FFEPSRGEKE   1050
VHGEFEKLLS TMQERFAEST D                                  1071