SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameVID24
Systematic nameYBR105C
AliasGID4
DescriptionVacuolar import and degradation protein 24

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI    RPN10 Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP38263
SGD YBR105C
Gene Ontology (GO)
Cellular componentGO:0016023cytoplasmic membrane-bound vesicleIDA:SGD.
Cellular componentGO:0019898extrinsic to membraneIDA:SGD.
Biological processGO:0045721negative regulation of gluconeogenesisIMP:SGD.
Biological processGO:0016192vesicle-mediated transportIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length362
Molecular weight41245

	---------+----------+----------+----------+----------+
MINNPKVDSV AEKPKAVTSK QSEQAASPEP TPAPPVSRNQ YPITFNLTST   50
APFHLHDRHR YLQEQDLYKC ASRDSLSSLQ QLAHTPNGST RKKYIVEDQS   100
PYSSENPVIV TSSYNHTVCT NYLRPRMQFT GYQISGYKRY QVTVNLKTVD   150
LPKKDCTSLS PHLSGFLSIR GLTNQHPEIS TYFEAYAVNH KELGFLSSSW   200
KDEPVLNEFK ATDQTDLEHW INFPSFRQLF LMSQKNGLNS TDDNGTTNAA   250
KKLPPQQLPT TPSADAGNIS RIFSQEKQFD NYLNERFIFM KWKEKFLVPD   300
ALLMEGVDGA SYDGFYYIVH DQVTGNIQGF YYHQDAEKFQ QLELVPSLKN   350
KVESSDCSFE FA                                            362