SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameVHS2
Systematic nameYIL135C
DescriptionProtein VHS2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI    RPN10 Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]

Cross References
Swiss-Prot accessionP40463
SGD YIL135C
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Biological processGO:0000082G1/S transition of mitotic cell cycleIGI:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
61Phosphoserine.
84Phosphoserine.
87Phosphoserine.
88Phosphoserine.
159Phosphoserine.
172Phosphoserine.
175Phosphoserine.
177Phosphoserine.
277Phosphoserine.
279Phosphothreonine.
303Phosphoserine.
325Phosphoserine.

Sequence Information
Sequence length436
Molecular weight47961

	---------+----------+----------+----------+----------+
MDTSNHNQDH DSHVAAQREN DNNYMPPSPS MSESSMIFER NVEDPSYLYK   50
TVSNNAANSL SRQSSRTSLF NHNNSSNRNF HNLSQRSSAV NLHLQPSRTN   100
ESIASYQTYN PDFVVQTPLD HRRTLENFVP PALDAGCSIV TDDTTGLDDV   150
DMVYSRRPST IGLDRALGRT RSLSSQSFDN ETSPAHPRSP NDHGSRLLRF   200
YSYADMLSDD NNNNVSNATS TSSTANPLRR PPMQGHYSFS SSLLNSPSHL   250
PSPPSASASP PQHMNFTNPF IISRRYSNTT INNANGGTSA GSTTGAALSR   300
SPSNQQYLLK QQRSPSGSAR SRRNSNRPGS AANIMIGKPK SKFHMESSGS   350
EGFSSEEEDN TMIERDKLNL KQKLQSQLAQ PPSIANMVND NHNNTNKHKN   400
TINNNIKNSP AFTNSNPSSK SNSNSTITSM NPDTTK                  436