SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameVAS1
Systematic nameYGR094W
DescriptionValyl-tRNA synthetase, mitochondrial

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
2        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[2]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP07806
SGD YGR094W
Gene Ontology (GO)
Cellular componentGO:0005739mitochondrionIDA:SGD.
Molecular functionGO:0042802identical protein bindingIPI:IntAct.
Biological processGO:0006438valyl-tRNA aminoacylationIDA:SGD.
EC number 6.1.1.9

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length1104
Molecular weight125770

	---------+----------+----------+----------+----------+
MNKWLNTLSK TFTFRLLNCH YRRSLPLCQN FSLKKSLTHN QVRFFKMSDL   50
DNLPPVDPKT GEVIINPLKE DGSPKTPKEI EKEKKKAEKL LKFAAKQAKK   100
NAAATTGASQ KKPKKKKEVE PIPEFIDKTV PGEKKILVSL DDPALKAYNP   150
ANVESSWYDW WIKTGVFEPE FTADGKVKPE GVFCIPAPPP NVTGALHIGH   200
ALTIAIQDSL IRYNRMKGKT VLFLPGFDHA GIATQSVVEK QIWAKDRKTR   250
HDYGREAFVG KVWEWKEEYH SRIKNQIQKL GASYDWSREA FTLSPELTKS   300
VEEAFVRLHD EGVIYRASRL VNWSVKLNTA ISNLEVENKD VKSRTLLSVP   350
GYDEKVEFGV LTSFAYPVIG SDEKLIIATT RPETIFGDTA VAVHPDDDRY   400
KHLHGKFIQH PFLPRKIPII TDKEAVDMEF GTGAVKITPA HDQNDYNTGK   450
RHNLEFINIL TDDGLLNEEC GPEWQGMKRF DARKKVIEQL KEKNLYVGQE   500
DNEMTIPTCS RSGDIIEPLL KPQWWVSQSE MAKDAIKVVR DGQITITPKS   550
SEAEYFHWLG NIQDWCISRQ LWWGHRCPVY FINIEGEEHD RIDGDYWVAG   600
RSMEEAEKKA AAKYPNSKFT LEQDEDVLDT WFSSGLWPFS TLGWPEKTKD   650
METFYPFSML ETGWDILFFW VTRMILLGLK LTGSVPFKEV FCHSLVRDAQ   700
GRKMSKSLGN VIDPLDVITG IKLDDLHAKL LQGNLDPREV EKAKIGQKES   750
YPNGIPQCGT DAMRFALCAY TTGGRDINLD ILRVEGYRKF CNKIYQATKF   800
ALMRLGDDYQ PPATEGLSGN ESLVEKWILH KLTETSKIVN EALDKRDFLT   850
STSSIYEFWY LICDVYIENS KYLIQEGSAI EKKSAKDTLY ILLDNALKLI   900
HPFMPFISEE MWQRLPKRST EKAASIVKAS YPVYVSEYDD VKSANAYDLV   950
LNITKEARSL LSEYNILKNG KVFVESNHEE YFKTAEDQKD SIVSLIKAID   1000
EVTVVRDASE IPEGCVLQSV NPEVNVHLLV KGHVDIDAEI AKVQKKLEKA   1050
KKSKNGIEQT INSKDYETKA NTQAKEANKS KLDNTVAEIE GLEATIENLK   1100
RLKL                                                     1104