SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameUSA1
Systematic nameYML029W
DescriptionU1 SNP1-associating protein 1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionQ03714
SGD YML029W
Gene Ontology (GO)
Cellular componentGO:0005789endoplasmic reticulum membraneIPI:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0030433ER-associated protein catabolic processIGI:SGD.
Biological processGO:0000398nuclear mRNA splicing, via spliceosomeIPI:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
376Phosphoserine.
379Phosphoserine.

Sequence Information
Sequence length838
Molecular weight96653

	---------+----------+----------+----------+----------+
MSEYLAQTPC KFTIWSSEID LIRTNLLVNA HPLSTVGRLL QYIHYQIYKQ   50
LRAIYQPEEQ CTNSEIPHTP LNSINTYFLS YEGRELSATC LLKDITSSSH   100
PDSNHFIRLQ LEKRTSPSGS AFDLEYDMEG EFNSMNIQFE INTLSSQRIF   150
NSMEPNLPIG TTLARLEKLA LERIKDFEKS AGNLCGIKED HSVSDLQGFI   200
IKGKQTPMFL NYGSDSDYYK DLNLVDLIGI DFAPAHNSFF TFLFKMNHEQ   250
NSHIANDEER FVLEFISDAT LSITQMNVKP DTTVKQVKDF ICSVYTHSLN   300
LRRNDIKLIY KGQLLHENNF AGNSSKISEY IKEPHEVKVH VQINQEYTES   350
GPGFWNEVFN NPNIFQFMPP DTRSQSPVSF APTQGRSPAA IRGEERGIPY   400
VTESGNDIVP TDELYRKCII NGDEVVFIPV SELNPQSSYL SVIKGDYGEI   450
KIPISSNDYR INGDNILLSP SAIEQLESAL NFKIERPRDS TLLHPSGEHV   500
RAADNTSSAN DNNTVENDES AWNRRVVRPL RNSFPLLLVL IRTFYLIGYN   550
SLVPFFIILE FGSFLPWKYI ILLSLLFIFR TVWNTQEVWN LWRDYLHLNE   600
IDEVKFSQIK EFINSNSLTL NFYKKCKDTQ SAIDLLMIPN LHEQRLSVYS   650
KYDIEYDTNT PDVGQLNLLF IKVLSGEIPK DALDELFKEF FELYETTRNM   700
NTLYPQDSLN ELLLMIWKES QKKDINTLPK YRRWFQTLCS QIAEHNVLDV   750
VLRYIIPDPV NDRVITAVIK NFVLFWVTLL PYVKEKLDDI VAQRARDREQ   800
PAPSAQQQEN EDEALIIPDE EEPTATGAQP HLYIPDED                838