SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameURA7
Systematic nameYBL039C
DescriptionCTP synthase 1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1K422        
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"A proteomics approach to understanding protein ubiquitination."
Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
Nat Biotechnol. 21(8):921-6 (2003) [12872131]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP28274
SGD YBL039C
Gene Ontology (GO)
Cellular componentGO:0005829cytosolTAS:SGD.
Molecular functionGO:0003883CTP synthase activityIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0006241CTP biosynthetic processIDA:SGD.
Biological processGO:0008654phospholipid biosynthetic processIMP:SGD.
Biological processGO:0019856pyrimidine base biosynthetic processIMP:SGD.
EC number 6.3.4.2

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length579
Molecular weight64710

	---------+----------+----------+----------+----------+
MKYVVVSGGV ISGIGKGVLA SSTGMLMKTL GLKVTSIKID PYMNIDAGTM   50
SPLEHGECFV LDDGGETDLD LGNYERYLGV TLTKDHNITT GKIYSHVIAK   100
ERKGDYLGKT VQIVPHLTNA IQDWIERVAK IPVDDTGMEP DVCIIELGGT   150
VGDIESAPFV EALRQFQFKV GKENFALIHV SLVPVIHGEQ KTKPTQAAIK   200
GLRSLGLVPD MIACRCSETL DKPTIDKIAM FCHVGPEQVV NVHDVNSTYH   250
VPLLLLEQKM IDYLHARLKL DEISLTEEEK QRGLELLSKW KATTGNFDES   300
METVKIALVG KYTNLKDSYL SVIKALEHSS MKCRRKLDIK WVEATDLEPE   350
AQESNKTKFH EAWNMVSTAD GILIPGGFGV RGTEGMVLAA RWARENHIPF   400
LGVCLGLQIA TIEFTRSVLG RKDSHSAEFY PDIDEKNHVV VFMPEIDKET   450
MGGSMRLGLR PTFFQNETEW SQIKKLYGDV SEVHERHRHR YEINPKMVDE   500
LENNGLIFVG KDDTGKRCEI LELKNHPYYI ATQYHPEYTS KVLDPSKPFL   550
GLVAASAGIL QDVIEGKYDL EAGENKFNF                          579

	* Ubiquitinated lysine is denoted as red color.