SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameURA3
Systematic nameYEL021W
DescriptionOrotidine 5'-phosphate decarboxylase

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1K93;K209;K253        
2         
3K93;K142;K176;K209        
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"A proteomics approach to understanding protein ubiquitination."
Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
Nat Biotechnol. 21(8):921-6 (2003) [12872131]
[2]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[3]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP03962
SGD YEL021W
Gene Ontology (GO)
Molecular functionGO:0004590orotidine-5'-phosphate decarboxylase activityIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0006207'de novo' pyrimidine base biosynthetic processTAS:SGD.
EC number 4.1.1.23

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length267
Molecular weight29240

	---------+----------+----------+----------+----------+
MSKATYKERA ATHPSPVAAK LFNIMHEKQT NLCASLDVRT TKELLELVEA   50
LGPKICLLKT HVDILTDFSM EGTVKPLKAL SAKYNFLLFE DRKFADIGNT   100
VKLQYSAGVY RIAEWADITN AHGVVGPGIV SGLKQAAEEV TKEPRGLLML   150
AELSCKGSLA TGEYTKGTVD IAKSDKDFVI GFIAQRDMGG RDEGYDWLIM   200
TPGVGLDDKG DALGQQYRTV DDVVSTGSDI IIVGRGLFAK GRDAKVEGER   250
YRKAGWEAYL RRCGQQN                                       267

	* Ubiquitinated lysine is denoted as red color.