SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


Main Search Enzymes Substrates Ubiquitination types BLAST Feedback HELP

Substrate Information

General Information
Standard nameURA2
Systematic nameYJL130C
DescriptionAspartate carbamoyltransferase

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI       
2         
3        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[3]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP07259
SGD YJL130C
Gene Ontology (GO)
Cellular componentGO:0016021integral to membraneIDA:SGD.
Cellular componentGO:0005739mitochondrionIDA:SGD.
Molecular functionGO:0004070aspartate carbamoyltransferase activityIDA:SGD.
Molecular functionGO:0004088carbamoyl-phosphate synthase (glutamine-hyd...IDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0006207'de novo' pyrimidine base biosynthetic processTAS:SGD.
Biological processGO:0006541glutamine metabolic processIDA:SGD.
EC number 6.3.5.5,2.1.3.2

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
1857Phosphoserine, by PKA.
1859Phosphothreonine.

Sequence Information
Sequence length2214
Molecular weight245126

	---------+----------+----------+----------+----------+
MATIAPTAPI TPPMESTGDR LVTLELKDGT VLQGYSFGAE KSVAGELVFQ   50
TGMVGYPESV TDPSYEGQIL VITYPLVGNY GVPDMHLRDE LVEELPRYFE   100
SNRIHIAGLV ISHYTDEYSH YLRKSSLGKW LQNEGIPAVY GVDTRSLTKH   150
LRDAGSMLGR LSLEKSGSDR TISRSSSWRS AFDVPEWVDP NVQNLVSKVS   200
INEPKLYVPP ADNKHIELQT GPDGKVLRIL AIDVGMKYNQ IRCFIKRGVE   250
LKVVPWNYDF TKEDYDGLFI SNGPGDPSVL DDLSQRLSNV LEAKKTPVFG   300
ICLGHQLIAR AAGASTLKLK FGNRGHNIPC TSTISGRCYI TSQNHGFAVD   350
VDTLTSGWKP LFVNANDDSN EGIYHSELPY FSVQFHPEST PGPRDTEFLF   400
DVFIQAVKEF KYTQVLKPIA FPGGLLEDNV KAHPRIEAKK VLVLGSGGLS   450
IGQAGEFDYS GSQAIKALKE EGIYTILINP NIATIQTSKG LADKVYFVPV   500
TAEFVRKVIL HERPDAIYVT FGGQTALSVG IAMKDEFEAL GVKVLGTPID   550
TIITTEDREL FSNAIDEINE KCAKSQAANS VDEALAAVKE IGFPVIVRAA   600
YALGGLGSGF ANNEKELVDL CNVAFSSSPQ VLVEKSMKGW KEVEYEVVRD   650
AFDNCITVCN MENFDPLGIH TGDSIVVAPS QTLSDEDYNM LRTTAVNVIR   700
HLGVVGECNI QYALNPVSKD YCIIEVNARL SRSSALASKA TGYPLAYTAA   750
KLGLNIPLNE VKNSVTKSTC ACFEPSLDYC VVKMPRWDLK KFTRVSTELS   800
SSMKSVGEVM SIGRTFEEAI QKAIRSTEYA NLGFNETDLD IDIDYELNNP   850
TDMRVFAIAN AFAKKGYSVD KVWEMTRIDK WFLNKLHDLV QFAEKISSFG   900
TKEELPSLVL RQAKQLGFDD RQIARFLDSN EVAIRRLRKE YGITPFVKQI   950
DTVAAEFPAY TNYLYMTYNA DSHDLSFDDH GVMVLGSGVY RIGSSVEFDW   1000
CAVTAVRTLR ANNIKTIMVN YNPETVSTDY DEADRLYFET INLERVLDIY   1050
EIENSSGVVV SMGGQTSNNI AMTLHRENVK ILGTSPDMID SAENRYKFSR   1100
MLDQIGVDQP AWKELTSMDE AESFAEKVGY PVLVRPSYVL SGAAMNTVYS   1150
KNDLESYLNQ AVEVSRDYPV VITKYIENAK EIEMDAVARN GELVMHVVSE   1200
HVENAGVHSG DATLIVPPQD LAPETVDRIV VATAKIGKAL KITGPYNIQF   1250
IAKDNEIKVI ECNVRASRSF PFISKVVGVN LIELATKAIM GLPLTPYPVE   1300
KLPDDYVAVK VPQFSFPRLA GADPVLGVEM ASTGEVATFG HSKYEAYLKS   1350
LLATGFKLPK KNILLSIGSY KEKQELLSSV QKLYNMGYKL FATSGTADFL   1400
SEHGIAVQYL EVLNKDDDDQ KSEYSLTQHL ANNEIDLYIN LPSANRFRRP   1450
ASYVSKGYKT RRLAVDYSVP LVTNVKCAKL LIEAISRNIT LDVSERDAQT   1500
SHRTITLPGL INIATYVPNA SHVIKGPAEL KETTRLFLES GFTYCQLMPR   1550
SISGPVITDV ASLKAANSVS QDSSYTDFSF TIAGTAHNAH SVTQSASKVT   1600
ALFLPLRELK NKITAVAELL NQWPTEKQVI AEAKTADLAS VLLLTSLQNR   1650
SIHITGVSNK EDLALIMTVK AKDPRVTCDV NIYSLFIAQD DYPEAVFLPT   1700
KEDQEFFWNN LDSIDAFSVG ALPVALANVT GNKVDVGMGI KDSLPLLLAA   1750
VEEGKLTIDD IVLRLHDNPA KIFNIPTQDS VVEIDLDYSF RRNKRWSPFN   1800
KDMNGGIERV VYNGETLVLS GELVSPGAKG KCIVNPSPAS ITASAELQST   1850
SAKRRFSITE EAIADNLDAA EDAIPEQPLE QKLMSSRPPR ELVAPGAIQN   1900
LIRSNNPFRG RHILSIKQFK RSDFHVLFAV AQELRAAVAR EGVLDLMKGH   1950
VITTIFFEPS TRTCSSFIAA MERLGGRIVN VNPLVSSVKK GETLQDTIRT   2000
LACYSDAIVM RHSEEMSVHI AAKYSPVPII NGGNGSREHP TQAFLDLFTI   2050
REEIGTVNGI TVTFMGDLKH GRTVHSLCRL LMHYQVRINL VSPPELRLPE   2100
GLREELRKAG LLGVESIELT PHIISKTDVL YCTRVQEERF NSPEEYARLK   2150
DTYIVDNKIL AHAKENMAIM HPLPRVNEIK EEVDYDHRAA YFRQMKYGLF   2200
VRMALLAMVM GVDM                                          2214