SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameUPF3
Systematic nameYGR072W
AliasSUA6
DescriptionNonsense-mediated mRNA decay protein 3

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1K331        
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP48412
SGD YGR072W
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmTAS:SGD.
Cellular componentGO:0005634nucleusIDA:SGD.
Cellular componentGO:0005844polysomeIDA:SGD.
Biological processGO:0000184mRNA catabolic process, nonsense-mediated d...TAS:SGD.
Biological processGO:0000723telomere maintenanceIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length387
Molecular weight44924

	---------+----------+----------+----------+----------+
MSNVAGELKN SEGKKKGRGN RYHNKNRGKS KNETVDPKKN ENKVNNATNA   50
THNNSKGRRN NKKRNREYYN YKRKARLGKS TENEGFKLVI RLLPPNLTAD   100
EFFAILRDNN NDDGDKQDIQ GKLKYSDWCF FEGHYSSKVF KNSTYSRCNF   150
LFDNLSDLEK CANFIKTCKF IDNKDNITIP DMKLSPYVKK FTQTSKKDAA   200
LVGTIEEDEI FKTFMNSMKQ LNENDEYSFQ DFSVLKSLEK EFSKSIELEN   250
KIAERTERVL TELVGTGDKV KNKNKKKKNK NAKKKFKEEE ASAKIPKKKR   300
NRGKKKRENR EKSTISKTKN SNVVIIEEAG KEVLKQRKKK MLLQEKLKIS   350
NSSQPQSSSA QTQPSFQPKE NLFVPRVKIL HRDDTKK                 387

	* Ubiquitinated lysine is denoted as red color.