SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameUGA3
Systematic nameYDL170W
DescriptionTranscriptional activator protein UGA3

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP26370
SGD YDL170W
Gene Ontology (GO)
Cellular componentGO:0005634nucleusIC:SGD.
Molecular functionGO:0003704specific RNA polymerase II transcription fa...IPI:SGD.
Molecular functionGO:0003700transcription factor activityIDA:SGD.
Biological processGO:0019740nitrogen utilizationIMP:SGD.
Biological processGO:0045893positive regulation of transcription, DNA-d...IDA:SGD.
Biological processGO:0006357regulation of transcription from RNA polyme...IMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length528
Molecular weight61224

	---------+----------+----------+----------+----------+
MNYGVEKLKL KYSKHGCITC KIRKKRCSED KPVCRDCRRL SFPCIYISES   50
VDKQSLKKIK ADIQHQLISK KRKHAPDSAQ KAAVATRTRR VGSDEQDNQV   100
YLSKPLEDCI SQKLDSMGLQ LYNYYRSHLA NIISIAPMNQ NYYLNIFLPM   150
AHENDGILFA ILAWSANHLS ISSSNELRKD EIFVNLANKY TYMSLSHLKT   200
NEGSSACAKL GFLYSLAQIL ILCGSEICQG DVKFWKILLN IGKNLIENHV   250
GKDVSRILTT TTEEPSLEER IIFPNFNSVV KYWLIVNFIY HDILNFNTTS   300
FPIEQYEKFF QRDQNSLPSS ANFIESIDSP IEEIDPLIGI NKPILLLLGQ   350
VTNLTRFLQT MEQEEMLEHG DKILSLQVEI YKLQPSLMAL EHLDDEKKFY   400
YLELFEIMKI STLMFFQLTL LKIDKDSLEL QILRNKLDSK LDKVIGTFLE   450
GSLCFPLFIY GVCIQVEDME KKIDLEAKFD DILKRYKCYN FQNARLLIRK   500
IWQNEADGIS EHDLVHMIDE LDYNINFA                           528