SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameUFO1
Systematic nameYML088W
DescriptionUbiquitin ligase complex F-box protein UFO1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 AUTOCDC34RINGSCFUFO1DDI1 Proteasomal Degradation
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidenceHigh MW Product Confirmation
MethodWestern blot (in vitro)
Reference"Functional interaction of 13 yeast SCF complexes with a set of yeast E2 enzymes in vitro."
Kus BM, Caldon CE, Andorn-Broza R, Edwards AM.
Proteins. 54(3):455-67 (2004) [14747994]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionQ04511
SGD YML088W
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Cellular componentGO:0005634nucleusIDA:SGD.
Cellular componentGO:0019005SCF ubiquitin ligase complexIPI:SGD.
Molecular functionGO:0005515protein bindingIDA:SGD.
Molecular functionGO:0004842ubiquitin-protein ligase activityIMP:SGD.
Biological processGO:0006974response to DNA damage stimulusIMP:SGD.
Biological processGO:0006511ubiquitin-dependent protein catabolic processIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length668
Molecular weight76860

	---------+----------+----------+----------+----------+
MERPGLVLQD LPPEILINIF SHLDEKDLFT LQELSTHFRN LIHDEELWKN   50
LFKSRVHTTH FPTFSQSSKF SVEYIERTRG LHHWQHNKAI RTKYTIIPTR   100
NWDQPSIERI VFDYPRVAAY NDGTITILQL QNHKRQKKFK KLIYIPCTTP   150
QGCSTMDFNI NAAVFGRFDG RVFGKLLSNK SYLTPVMEFT GRHSAGVTAI   200
CNSESWDTSR EDWSVSGSEN GEIIWWCENK LVKMWKVSNR VIWKLAFFKD   250
WTLIMDDEKL YIIHQMQELH SIDIPKDLDE QPMRVRFFKM DFGSMTLVLA   300
DLNNVYTISV NPNGNFGNLR KLEMPEQICA VEIDEKTSQR EQNWQFAGDD   350
GCYISLLTTQ NTLYIINIRD LSSSGLKVQC KISFDEQVYV SQVTNLIVVV   400
ALPNVLQILN AMTGELIKTV LKTEKFPEFL KVSQDKIIMG SGNVLNYLKF   450
VSSDSKKHHH STKGKNTVSN KWNETLNTEL QYYDEDEDLR RKRQSEISRL   500
IDAYGGDLEL SGDTDEENDI QLRIALLESQ EAQARNQAEA GEPVGDDEDE   550
QLRRALEESQ LIYETQTNSS ANHGNNTNDE IDEDDEEFLR AIRQSRVEDE   600
RRRHLRNHTT GRRNGPLSDD NFATYGAAES SERTSTENTI GSSVGVDASN   650
NVDEDLQLAI ALSLSEIN                                      668