SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameUFD2
Systematic nameYDL190C
DescriptionUbiquitin conjugation factor E4

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI       
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]

Cross References
Swiss-Prot accessionP54860
SGD YDL190C
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Cellular componentGO:0005634nucleusIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Molecular functionGO:0004842ubiquitin-protein ligase activityIMP:SGD.
Biological processGO:0006950response to stressIMP:SGD.
Biological processGO:0006512ubiquitin cycleIGI:SGD.
Biological processGO:0006511ubiquitin-dependent protein catabolic processIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length961
Molecular weight109906

	---------+----------+----------+----------+----------+
MTAIEDILQI TTDPSDTRGY SLLKSEEVPQ GSTLGVDFID TLLLYQLTEN   50
EKLDKPFEYL NDCFRRNQQQ KRITKNKPNA ESLHSTFQEI DRLVIGYGVV   100
ASQIENFCMN GAFINYITGI VSNVNSYTDF LSQIIQRAIL EGTALDLLNA   150
VFPTLLEYCN KHVSHFDLNE SVIYNNVLTI FELFVTFKPI AEIFTKIDGF   200
FADYSCKPQD FERKTILGPI LSLSPIEAAV AIRNYGDNLL RSKQQTAMIH   250
ESLQAEHKVV IDRLFFIVDK LVRGSLNSRT DMISYFAHIA NKNHLRRADH   300
PPFKELSSNG FMSNITLLLV RFSQPFLDIS YKKIDKIDAN YFNNPSLFID   350
LSGETRLNSD FKEADAFYDK NRKTADSKPN FISDCFFLTL TYLHYGLGGT   400
LSFEEKMGSE IKALKEEIEK VKKIAANHDV FARFITAQLS KMEKALKTTE   450
SLRFALQGFF AHRSLQLEVF DFICGASTFL IRVVDPEHEF PFKQIKLPLI   500
PDQIGVENVD NADFLRAHAP VPFKYYPEFV VEGPVNYSLY ISKYQTSPIF   550
RNPRLGSFVE FTTMVLRCPE LVSNPHLKGK LVQLLSVGAM PLTDNSPGFM   600
MDIFEHDELV NKNLLYALLD FYVIVEKTGS SSQFYDKFNS RYSISIILEE   650
LYYKIPSYKN QLIWQSQNNA DFFVRFDARM LNDLTFLLDE GLSNLAEVHN   700
IQNELDNRAR GAPPTREEED KELQTRLASA SRQAKSSCGL ADKSMKLFEI   750
YSKDIPAAFV TPEIVYRLAS MLNYNLESLV GPKCGELKVK DPQSYSFNPK   800
DLLKALTTVY INLSEQSEFI SAVAKDERSF NRNLFVRAVD ILGRKTGLAS   850
PEFIEKLLNF ANKAEEQRKA DEEEDLEYGD VPDEFLDPLM YTIMKDPVIL   900
PASKMNIDRS TIKAHLLSDS TDPFNRMPLK LEDVTPNEEL RQKILCFKKQ   950
KKEEAKHKAS E                                             961