SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameUBX7
Systematic nameYBR273C
DescriptionUBX domain-containing protein 7

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI    RPN10 Proteasomal Degradation
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP38349
SGD YBR273C
Gene Ontology (GO)
Cellular componentGO:0005783endoplasmic reticulumIDA:SGD.
Cellular componentGO:0005635nuclear envelopeIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0030435sporulationIMP:SGD.
Biological processGO:0006511ubiquitin-dependent protein catabolic processIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
349Phosphothreonine.

Sequence Information
Sequence length436
Molecular weight50031

	---------+----------+----------+----------+----------+
MLEALFRDSV EEAINDSIKE GVVLAVYNTA RDDQWLKSWF KGDDVSLDTL   50
AEHSIWLRLV KDTEQFQLFE QVFPNVVVPS IYLIRAGKIE LIIQGEDDRH   100
WEKLLACIGI KDKKAGESSS RETNPGLARE EKSSRDVHRK NARERIAETT   150
LEIQRREQLK QRKLAEEERE RIIRLVRADR AERKALDETH HRTLDDDKPL   200
DVHDYIKDAQ KLHSSKCVLQ IRMTDGKTLK HEFNSSETLN DVRKWVDVNR   250
TDGDCPYSFH RGIPRVTFKD SDELKTLETL ELTPRSALLL KPLETQNSGL   300
SVTGMEGPSL LGRLYKGFST WWHNDKDPEV TSQREETSKP NRHEVRSSTP   350
LSGAASSSCF QYNNVREPVQ SSAHASPMLT PSGTRYPSET NLTTSRSVSP   400
NVFQFVNNDH QEDPEDPTTF NGNNVHLEKK KDEDKK                  436